PDBsum entry: 3bvw

Hydrolase

PDB-id: 3bvw

Contents

Description

Header details

Header records

PROCHECK

Protein chain

1015 a.a. *

Ligands

WZ4

MPD

Metal ions

_ZN

Waters ×1403

* Residue conservation analysis

Tools

Clefts Calculation

PDB id:

3bvw

Name:

Hydrolase

Title:

Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl (2-deoxy-2-acetamido-beta-d- glucopyranosyl)-(1->2)-alpha-d-mannopyranosyl- (1->3)- [alpha-d-mannopyranosyl-(1->6)-6-thio-alpha-d- mannopyranosyl- (1->6)]-beta-d-mannopyranoside

Structure:

Alpha-mannosidase 2. Chain: a. Fragment: catalytic domain. Unp residues 76-1108. Synonym: alpha-mannosidase ii, mannosyl-oligosaccharide 1, 3-1,6-alpha-mannosidase, man ii, golgi alpha-mannosidase ii, aman ii. Engineered: yes. Mutation: yes

Source:

Drosophila melanogaster. Fruit fly. Gene: alpha-man-ii, gmii. Expressed in: drosophila melanogaster.

UniProt:

Q24451 (MAN2_DROME)

Seq:

Struc:

Seq:

Struc:

Seq:

Struc:

Seq:

Struc:

Seq:

1108 a.a.

Struc:

1015 a.a.*

Key:	PfamA domain	PfamB domain
Secondary structure	CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme class:

E.C.3.2.1.114   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Hydrolysis of the terminal 1,3- and 1,6-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man(5)(GlcNAc)(3).

Pathway:

Mannosyl-glycoprotein N-acetylglucosaminyltransferases

Resolution:

1.20Å

R-factor:

0.133

R-free:

0.170

Authors:

D.A.Kuntz,D.R.Rose

Date:

07-Jan-08

Release date:

01-Jul-08

Related entries:

1hty
dgmii + tris
1hww
dgmii + swainsonine
1hxk
dgmii + deoxymannojirimicin
1ps2
dgmii + kifunensine
1qwn
dgmii + 5fluoro-gulosylfluoride
... plus others (see Header records)

Quick_links

Procheck

Clefts

Surface