PDBsum entry: 3bvv

Hydrolase

PDB id: 3bvv

Contents

Protein chain

1016 a.a. *

Ligands

PO4

MRD

WZ3

MPD ×2

Metals

_ZN

Waters ×1690

* Residue conservation analysis

PDB id:

3bvv

Name:

Hydrolase

Title:

Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl alpha-d-mannopyranosyl-(1->3)-[6-thio- alpha-d-mannopyranosyl-(1->6)]-beta-d-mannopyranoside

Structure:

Alpha-mannosidase 2. Chain: a. Fragment: catalytic domain. Unp residues 76-1108. Synonym: alpha-mannosidase ii, mannosyl-oligosaccharide 1, 3-1,6-alpha-mannosidase, man ii, golgi alpha-mannosidase ii, aman ii. Engineered: yes. Mutation: yes

Source:

Drosophila melanogaster. Fruit fly. Gene: alpha-man-ii, gmii. Expressed in: drosophila melanogaster.

Resolution:

1.30Å R-factor: 0.153 R-free: 0.173

Authors:

D.A.Kuntz,D.R.Rose

Date:

07-Jan-08 Release date: 01-Jul-08

Protein chain

Q24451  (MAN2_DROME) -  Alpha-mannosidase 2

Seq: 1108 a.a.

Struc: 1016 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.114 - Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase.
• Pathway: Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• Reaction: Hydrolysis of the terminal 1,3- and 1,6-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man(5)(GlcNAc)(3).

 Gene Ontology (GO) functional annotation 

• Cellular component: membrane (6 terms)
• Biological process: metabolic process (3 terms)
• Biochemical function: catalytic activity (11 terms)