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PDBsum entry 3brh

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3brh
Jmol
Contents
Protein chains
296 a.a. *
Ligands
ASP-ASN ×2
GLU ×2
TYR-THR-ALA-ARG
TYR
PO4 ×2
Waters ×295
* Residue conservation analysis
PDB id:
3brh
Name: Hydrolase
Title: Protein tyrosine phosphatase ptpn-22 (lyp) bound to the mono-phosphorylated lck active site peptide
Structure: Tyrosine-protein phosphatase non-receptor type 22. Chain: a, b. Fragment: substrate trapped catalytic domain. Synonym: hematopoietic cell protein-tyrosine phosphatase 70z-pep, lymphoid phosphatase, lyp. Engineered: yes. Mutation: yes. Lck active site peptide.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ptpn22, ptpn8. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: the peptide was chemically synthesized. The sequence of the peptide is naturally found in home
Resolution:
2.20Å     R-factor:   0.191     R-free:   0.264
Authors: R.D.Seidel,J.Love,A.Piserchio,D.Cowburn
Key ref: R.Seidel et al. Lyp/ptpn22 phosphatase domain: substrate recognition and specificity for src family kinases. To be published, .
Date:
21-Dec-07     Release date:   17-Feb-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q9Y2R2  (PTN22_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 22
Seq:
Struc:
 
Seq:
Struc:
807 a.a.
296 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.1.3.48  - Protein-tyrosine-phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate
Protein tyrosine phosphate
+ H(2)O
=
protein tyrosine
Bound ligand (Het Group name = TYR)
matches with 64.00% similarity
+
phosphate
Bound ligand (Het Group name = PO4)
corresponds exactly
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     dephosphorylation   2 terms 
  Biochemical function     phosphatase activity     2 terms