PDBsum entry: 3blb

Hydrolase

PDB id: 3blb

Contents

Protein chain

1014 a.a. *

Ligands

NAG

SWA

MRD

Metals

_ZN

Waters ×1068

* Residue conservation analysis

PDB id:

3blb

Name:

Hydrolase

Title:

Crystal structure of golgi mannosidase ii in complex with sw at 1.3 angstrom resolution

Structure:

Alpha-mannosidase 2. Chain: a. Fragment: catalytic domain: residues 76-1108. Synonym: alpha-mannosidase ii, mannosyl-oligosaccharide 1,3 alpha-mannosidase, man ii, golgi alpha-mannosidase ii, aman engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Strain: berkeley. Tissue: head. Gene: alpha-man-ii, gmii, cg18802. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.

Resolution:

1.30Å R-factor: 0.166 R-free: 0.185

Authors:

D.A.Kuntz,D.R.Rose

Key ref:

D.A.Kuntz and d.r.rose Golgi mannosidase ii in complex with swainsonine at 1.3 angstrom.. To be published,

Date:

10-Dec-07 Release date: 08-Jan-08

Protein chain

Q24451  (MAN2_DROME) -  Alpha-mannosidase 2

Seq: 1108 a.a.

Struc: 1014 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.2.1.114 - Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase.
• Pathway: Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• Reaction: Hydrolysis of the terminal 1,3- and 1,6-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man(5)(GlcNAc)(3).

 Gene Ontology (GO) functional annotation 

• Cellular component: membrane (6 terms)
• Biological process: metabolic process (3 terms)
• Biochemical function: catalytic activity (11 terms)