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PDBsum entry 3bdf

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3bdf
Jmol
Contents
Protein chains
434 a.a. *
Ligands
SO4 ×4
Waters ×1222
* Residue conservation analysis
PDB id:
3bdf
Name: Hydrolase
Title: Crystal structure of metal-free e. Coli alkaline phosphatase (t155v)
Structure: Alkaline phosphatase. Chain: a, b. Synonym: apase. Engineered: yes. Mutation: yes
Source: Escherichia coli. Strain: k12. Gene: phoa. Expressed in: escherichia coli.
Resolution:
1.40Å     R-factor:   0.163     R-free:   0.184
Authors: J.C.Grigg,M.E.Murphy
Key ref: J.C.Grigg et al. The active-Site trimetallic cluster of alkaline phosphatase is lost upon isosteric mutation at the mg2+-Coordinating residue threonine-155. To be published, .
Date:
14-Nov-07     Release date:   25-Nov-08    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00634  (PPB_ECOLI) -  Alkaline phosphatase
Seq:
Struc:
471 a.a.
434 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.3.1  - Alkaline phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: A phosphate monoester + H2O = an alcohol + phosphate
phosphate monoester
+ H(2)O
= alcohol
+ phosphate
      Cofactor: Mg(2+); Zn(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     periplasmic space   2 terms 
  Biological process     metabolic process   3 terms 
  Biochemical function     catalytic activity     10 terms