PDBsum entry: 3bac

Ligase

PDB-id: 3bac

Contents

Description

Header details

Header records

References

PROCHECK

Protein chain

255 a.a. *

Ligands

PO4 ×3

3B9

Waters ×15

* Residue conservation analysis

Tools

Clefts Calculation

PDB id:

3bac

Name:

Ligase

Title:

Structural basis for the inhibition of bacterial NAD+ dependent DNA ligase

Structure:

DNA ligase. Chain: a. Fragment: adenylation domain. Synonym: polydeoxyribonucleotide synthase [nad+]. Engineered: yes

Source:

Haemophilus influenzae. Organism_taxid: 727. Gene: lign, lig. Expressed in: escherichia coli. Expression_system_taxid: 562

UniProt:

P43813 (DNLJ_HAEIN)

Seq:

Struc:

Seq:

Struc:

Seq:

670 a.a.

Struc:

255 a.a.*

Key:

PfamA domain

Secondary structure

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme class:

E.C.6.5.1.2   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

NAD⁺ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (see diagram below)

Resolution:

3.00Å

R-factor:

0.216

R-free:

0.265

Authors:

C.Pinko

Key ref:

c.pinko et al. structural basis for the inhibition of bacterial nad+ dependent dna ligase. TO BE PUBLISHED, .

Date:

07-Nov-07

Release date:

11-Nov-08

Quick_links

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Clefts

Surface