PDBsum entry: 3bac

Ligase

PDB id

3bac

Contents

			Protein chain

					255 a.a. *

			Ligands

					PO4 ×3


					3B9

			Waters ×15

* Residue conservation analysis

PDB id:

3bac

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Name:

Ligase

Title:

Structural basis for the inhibition of bacterial NAD+ dependent DNA ligase

Structure:

DNA ligase. Chain: a. Fragment: adenylation domain. Synonym: polydeoxyribonucleotide synthase [nad+]. Engineered: yes

Source:

Haemophilus influenzae. Organism_taxid: 727. Gene: lign, lig. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

3.00Å

R-factor:

0.216

R-free:

0.265

Authors:

C.Pinko

Key ref:

C.Pinko et al. Structural basis for the inhibition of bacterial NAD+ dependent DNA ligase. To be published,

Date:

07-Nov-07

Release date:

11-Nov-08

PROCHECK

	Headers

	References

Protein chain

P43813 (DNLJ_HAEIN) - DNA ligase

Seq: Struc:

Seq: Struc:					670 a.a. 255 a.a.*

Key:

PfamA domain

Secondary structure

* PDB and UniProt seqs differ at 3 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.6.5.1.2 - Dna ligase (NAD(+)).

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

NAD⁺ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m)

NAD(+)	+	(deoxyribonucleotide)(n)	+	(deoxyribonucleotide)(m)	=	AMP	+	nicotinamide nucleotide	+	(deoxyribonucleotide)(n+m)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site



		Gene Ontology (GO) functional annotation

Biological process	DNA repair	2 terms
Biochemical function	DNA ligase (NAD+) activity	1 term