PDBsum entry: 3baa

Ligase

PDB id

3baa

Contents

			Protein chain

					312 a.a. *

			Ligands

					SO4 ×4


					NMN


					3BA


					GOL ×2

			Waters ×269

* Residue conservation analysis

PDB id:

3baa

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Name:

Ligase

Title:

Structural basis for the inhibition of bacterial NAD+ depend ligase

Structure:

DNA ligase. Chain: a. Fragment: adenylation domain. Engineered: yes

Source:

Enterococcus faecalis. Streptococcus faecalis. Organism_taxid: 1351. Gene: liga. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.90Å

R-factor:

0.187

R-free:

0.216

Authors:

C.Pinko

Key ref:

C.Pinko et al. Structural basis for the inhibition of bacterial NAD+ dependent DNA ligase. To be published,

Date:

07-Nov-07

Release date:

11-Nov-08

PROCHECK

	Headers

	References

Protein chain

Q837V6 (DNLJ_ENTFA) - DNA ligase

Seq: Struc:

Seq: Struc:					676 a.a. 312 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.6.5.1.2 - Dna ligase (NAD(+)).

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

NAD⁺ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m)

NAD(+)

(deoxyribonucleotide)(n)

(deoxyribonucleotide)(m)

AMP

nicotinamide nucleotide
Bound ligand (Het Group name = NMN)
corresponds exactly

(deoxyribonucleotide)(n+m)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site



		Gene Ontology (GO) functional annotation

Biological process	DNA repair	2 terms
Biochemical function	DNA ligase (NAD+) activity	1 term