spacer
spacer

PDBsum entry 3ax3

Go to PDB code: 
protein Protein-protein interface(s) links
Membrane protein/transport protein PDB id
3ax3
Jmol
Contents
Protein chains
66 a.a.
12 a.a.
68 a.a.
Waters ×36
PDB id:
3ax3
Name: Membrane protein/transport protein
Title: Crystal structure of rat tom20-aldh presequence complex: a c (form2) between tom20 and a disulfide-bridged presequence p containing d-cys and l-cys at the i and i+3 positions.
Structure: Mitochondrial import receptor subunit tom20 homol chain: a, c, e, g. Fragment: cytosolic domain, unp residues 59-126. Synonym: mitochondrial 20 kda outer membrane protein, outer mitochondrial membrane receptor tom20. Engineered: yes. Mutation: yes. Aldehyde dehydrogenase, mitochondrial. Chain: b, d, f, h.
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: the peptide was chemically synthesized.
Resolution:
2.10Å     R-factor:   0.245     R-free:   0.287
Authors: T.Saitoh,Y.Maita,D.Kohda
Key ref: T.Saitoh et al. (2011). Crystallographic snapshots of Tom20-mitochondrial presequence interactions with disulfide-stabilized peptides. Biochemistry, 50, 5487-5496. PubMed id: 21591667 DOI: 10.1021/bi200470x
Date:
28-Mar-11     Release date:   06-Jul-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q62760  (TOM20_RAT) -  Mitochondrial import receptor subunit TOM20 homolog
Seq:
Struc:
145 a.a.
66 a.a.*
Protein chains
Pfam   ArchSchema ?
P11884  (ALDH2_RAT) -  Aldehyde dehydrogenase, mitochondrial
Seq:
Struc:
519 a.a.
12 a.a.*
Protein chains
Pfam   ArchSchema ?
Q62760  (TOM20_RAT) -  Mitochondrial import receptor subunit TOM20 homolog
Seq:
Struc:
145 a.a.
68 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains B, D, F, H: E.C.1.2.1.3  - Aldehyde dehydrogenase (NAD(+)).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: An aldehyde + NAD+ + H2O = a carboxylate + NADH
aldehyde
+ NAD(+)
+ H(2)O
= carboxylate
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     mitochondrial outer membrane translocase complex   1 term 
  Biological process     intracellular protein transport   2 terms 

 

 
    reference    
 
 
DOI no: 10.1021/bi200470x Biochemistry 50:5487-5496 (2011)
PubMed id: 21591667  
 
 
Crystallographic snapshots of Tom20-mitochondrial presequence interactions with disulfide-stabilized peptides.
T.Saitoh, M.Igura, Y.Miyazaki, T.Ose, N.Maita, D.Kohda.
 
  ABSTRACT  
 
No abstract given.