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PDBsum entry 3apc

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protein ligands links
Transferase/transferase inhibitor PDB id
3apc
Jmol
Contents
Protein chain
839 a.a.
Ligands
MMD
SO4 ×2
Waters ×22
PDB id:
3apc
Name: Transferase/transferase inhibitor
Title: Crystal structure of human pi3k-gamma in complex with ch5132
Structure: Phosphatidylinositol-4,5-bisphosphate 3-kinase ca subunit gamma isoform. Chain: a. Fragment: residues 144-1102. Synonym: pi3-kinase subunit gamma, pi3k-gamma, ptdins-3-kin subunit gamma, phosphatidylinositol-4,5-bisphosphate 3-kina catalytic subunit gamma, ptdins-3-kinase subunit p110-gamma pi3k. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: pik3cg. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.
Resolution:
2.54Å     R-factor:   0.239     R-free:   0.290
Authors: M.Nakamura,T.A.Fukami,T.Miyazaki,M.Yoshida
Key ref: J.Ohwada et al. (2011). Discovery and biological activity of a novel class I PI3K inhibitor, CH5132799. Bioorg Med Chem Lett, 21, 1767-1772. PubMed id: 21316229 DOI: 10.1016/j.bmcl.2011.01.065
Date:
14-Oct-10     Release date:   13-Apr-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P48736  (PK3CG_HUMAN) -  Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1102 a.a.
839 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: E.C.2.7.1.153  - Phosphatidylinositol-4,5-bisphosphate 3-kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
1-Phosphatidyl-myo-inositol Metabolism
      Reaction: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP
+ 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
= ADP
+ 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
   Enzyme class 3: E.C.2.7.11.1  - Non-specific serine/threonine protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+ protein
= ADP
+ phosphoprotein
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     phosphatidylinositol-mediated signaling   2 terms 
  Biochemical function     transferase activity, transferring phosphorus-containing groups     2 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.bmcl.2011.01.065 Bioorg Med Chem Lett 21:1767-1772 (2011)
PubMed id: 21316229  
 
 
Discovery and biological activity of a novel class I PI3K inhibitor, CH5132799.
J.Ohwada, H.Ebiike, H.Kawada, M.Tsukazaki, M.Nakamura, T.Miyazaki, K.Morikami, K.Yoshinari, M.Yoshida, O.Kondoh, S.Kuramoto, K.Ogawa, Y.Aoki, N.Shimma.
 
  ABSTRACT  
 
No abstract given.