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PDBsum entry 3amp

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3amp
Jmol
Contents
Protein chains
255 a.a.
Ligands
CTR ×2
CBI ×2
Waters ×536
PDB id:
3amp
Name: Hydrolase
Title: E134c-cellotetraose complex of cellulase 12a from thermotoga
Structure: Endo-1,4-beta-glucanase. Chain: a, b. Synonym: cellulase 12a. Engineered: yes. Mutation: yes
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: cela. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.78Å     R-factor:   0.169     R-free:   0.202
Authors: Y.-S.Cheng,T.-P.Ko,J.-R.Liu,R.-T.Guo
Key ref: Y.S.Cheng et al. (2011). Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima. Proteins, 79, 1193-1204. PubMed id: 21268113
Date:
20-Aug-10     Release date:   16-Mar-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q60032  (Q60032_THEMT) -  Endo-1,4-beta-glucanase (Precursor)
Seq:
Struc:
257 a.a.
255 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.4  - Cellulase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   2 terms 
  Biochemical function     hydrolase activity     4 terms  

 

 
Proteins 79:1193-1204 (2011)
PubMed id: 21268113  
 
 
Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima.
Y.S.Cheng, T.P.Ko, T.H.Wu, Y.Ma, C.H.Huang, H.L.Lai, A.H.Wang, J.R.Liu, R.T.Guo.
 
  ABSTRACT  
 
No abstract given.