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PDBsum entry 3aiq

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protein ligands links
Hydrolase PDB id
3aiq
Jmol
Contents
Protein chain
491 a.a. *
Ligands
HBO
Waters ×694
* Residue conservation analysis
PDB id:
3aiq
Name: Hydrolase
Title: Crystal structure of beta-glucosidase in wheat complexed wit aglycone dimboa
Structure: Beta-glucosidase. Chain: a. Fragment: residues in unp 50-569. Engineered: yes
Source: Triticum aestivum. Wheat. Organism_taxid: 4565. Gene: taglu1b. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.90Å     R-factor:   0.195     R-free:   0.211
Authors: M.Sue,C.Nakamura,T.Miyamoto,S.Yajima
Key ref: M.Sue et al. (2011). Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae. Plant Sci, 180, 268-275. PubMed id: 21421370 DOI: 10.1016/j.plantsci.2010.09.001
Date:
18-May-10     Release date:   23-Feb-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q1XH05  (Q1XH05_WHEAT) -  4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic
Seq:
Struc:
 
Seq:
Struc:
569 a.a.
491 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: E.C.3.2.1.182  - 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-7-methoxy-2H-1,4- benzoxazin-3(4H)-one + D-glucose
2. (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside + H2O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
+ H(2)O
= 2,4-dihydroxy-7-methoxy-2H-1,4- benzoxazin-3(4H)-one
+ D-glucose
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside
+ H(2)O
= 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
+ D-glucose
   Enzyme class 3: E.C.3.2.1.21  - Beta-glucosidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     plastid   2 terms 
  Biological process     metabolic process   2 terms 
  Biochemical function     hydrolase activity     4 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.plantsci.2010.09.001 Plant Sci 180:268-275 (2011)
PubMed id: 21421370  
 
 
Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae.
M.Sue, C.Nakamura, T.Miyamoto, S.Yajima.
 
  ABSTRACT  
 
No abstract given.