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PDBsum entry 3ag7

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protein links
Plant protein PDB id
3ag7
Jmol
Contents
Protein chain
105 a.a. *
Waters ×104
* Residue conservation analysis
PDB id:
3ag7
Name: Plant protein
Title: An auxilin-like j-domain containing protein, jac1 j-domain
Structure: Putative uncharacterized protein f9e10.5. Chain: a. Fragment: j-domain, unp residues 551-651. Synonym: at1g75100/f9e10_5, auxilin-like j-domain protein. Engineered: yes
Source: Arabidopsis thaliana. Mouse-ear cress, thale-cress. Organism_taxid: 3702. Strain: columbia. Gene: at1g75100, f9e10.5, jac1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.80Å     R-factor:   0.175     R-free:   0.231
Authors: A.Takano
Key ref: A.Takano et al. (2010). Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photorelocation movement in Arabidopsis thaliana. Plant Cell Physiol, 51, 1372-1376. PubMed id: 20562448
Date:
24-Mar-10     Release date:   13-Oct-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9C9Q4  (Q9C9Q4_ARATH) -  J domain-containing protein required for chloroplast accumulation response 1
Seq:
Struc:
 
Seq:
Struc:
651 a.a.
105 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 

 
Plant Cell Physiol 51:1372-1376 (2010)
PubMed id: 20562448  
 
 
Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photorelocation movement in Arabidopsis thaliana.
A.Takano, N.Suetsugu, M.Wada, D.Kohda.
 
  ABSTRACT  
 
An auxilin-like J-domain containing protein, JAC1, is necessary for chloroplast movement in Arabidopsis thaliana, to capture photosynthetic light efficiently under weak light conditions. Here, we performed crystallographic and functional analyses of the J-domain of JAC1. The crystal structure of the J-domain is quite similar to that of bovine auxilin, and possesses a similar positively charged surface, which probably forms the interface with the Hsp70 chaperone. The mutation of the highly-conserved HPD motif of the JAC1 J-domain abrogated the chloroplast photorelocation response. These results suggest that the requirement of JAC1 in chloroplast photorelocation movement is attributable to the J-domain's cochaperone activity.