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PDBsum entry 3aex

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protein ligands Protein-protein interface(s) links
Lyase PDB id
3aex
Jmol
Contents
Protein chains
351 a.a. *
Ligands
AN7 ×2
PO4 ×2
Waters ×252
* Residue conservation analysis
PDB id:
3aex
Name: Lyase
Title: Catalytic intermediate analogue of threonine synthase from t thermophilus hb8
Structure: Threonine synthase. Chain: a, b. Engineered: yes
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Gene: ttha0491. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.10Å     R-factor:   0.216     R-free:   0.253
Authors: T.Murakawa,Y.Machida,H.Hayashi
Key ref: T.Murakawa et al. (2011). Product-assisted catalysis as the basis of the reaction specificity of threonine synthase. J Biol Chem, 286, 2774-2784. PubMed id: 21084312 DOI: 10.1074/jbc.M110.186205
Date:
13-Feb-10     Release date:   17-Nov-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q5SL02  (Q5SL02_THET8) -  Threonine synthase
Seq:
Struc:
351 a.a.
351 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.2.3.1  - Threonine synthase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Threonine Biosynthesis
      Reaction: O-phospho-L-homoserine + H2O = L-threonine + phosphate
O-phospho-L-homoserine
+ H(2)O
= L-threonine
+ phosphate
      Cofactor: Pyridoxal 5'-phosphate
Pyridoxal 5'-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     cellular amino acid metabolic process   3 terms 
  Biochemical function     lyase activity     3 terms  

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M110.186205 J Biol Chem 286:2774-2784 (2011)
PubMed id: 21084312  
 
 
Product-assisted catalysis as the basis of the reaction specificity of threonine synthase.
T.Murakawa, Y.Machida, H.Hayashi.
 
  ABSTRACT  
 
No abstract given.