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PDBsum entry 3acg

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protein ligands metals links
Hydrolase PDB id
3acg
Jmol
Contents
Protein chain
193 a.a. *
Ligands
BGC-BGC
PO4 ×2
GOL
Metals
_CA
Waters ×413
* Residue conservation analysis
PDB id:
3acg
Name: Hydrolase
Title: Crystal structure of carbohydrate-binding module family 28 f clostridium josui cel5a in complex with cellobiose
Structure: Beta-1,4-endoglucanase. Chain: a. Fragment: unp residues 560-752. Synonym: carbohydrate-binding module family 28. Engineered: yes
Source: Clostridium josui. Organism_taxid: 1499. Gene: cela. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.50Å     R-factor:   0.149     R-free:   0.190
Authors: K.Tsukimoto,R.Takada,Y.Araki,K.Suzuki,S.Karita,T.Wakagi,H.Sh T.Watanabe,S.Fushinobu
Key ref: K.Tsukimoto et al. (2010). Recognition of cellooligosaccharides by a family 28 carbohydrate-binding module. FEBS Lett, 584, 1205-1211. PubMed id: 20159017 DOI: 10.1016/j.febslet.2010.02.027
Date:
04-Jan-10     Release date:   02-Mar-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q59290  (Q59290_CLOJO) -  Beta-1,4-endoglucanase
Seq:
Struc:
 
Seq:
Struc:
930 a.a.
193 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.4  - Cellulase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     cellulose catabolic process   1 term 
  Biochemical function     cellulase activity     1 term  

 

 
DOI no: 10.1016/j.febslet.2010.02.027 FEBS Lett 584:1205-1211 (2010)
PubMed id: 20159017  
 
 
Recognition of cellooligosaccharides by a family 28 carbohydrate-binding module.
K.Tsukimoto, R.Takada, Y.Araki, K.Suzuki, S.Karita, T.Wakagi, H.Shoun, T.Watanabe, S.Fushinobu.
 
  ABSTRACT  
 
The crystal structures of a carbohydrate-binding module (CBM) family 28 domain of endoglucanase Cel5A from Clostridium josui have been determined in ligand-free and complex forms with cellobiose, cellotetraose, and cellopentaose as the first complex structures of this family. In the cleft of a beta-sandwich fold, the ligands are recognized by stacking interactions and hydrogen bonds. Conformations of the bound cellooligosaccharides are similar to those in crystals and solution but clearly different from the cellulose structure. Interestingly, the glucan chain bound on CBM28 is in the opposite direction of that bound to CBM17, although these families share significant structural similarity.