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PDBsum entry 3a4c
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Cell cycle, replication PDB id
3a4c

 

 

 

 

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Contents
Protein chain
106 a.a. *
Waters ×78
* Residue conservation analysis
PDB id:
3a4c
Name: Cell cycle, replication
Title: Crystal structure of cdt1 c terminal domain
Structure: DNA replication factor cdt1. Chain: a. Fragment: c terminal domain, residues 452-557. Synonym: double parked homolog, dup, retroviral insertion site 2 protein. Engineered: yes
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: cdt1, ris2. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.89Å     R-factor:   0.213     R-free:   0.236
Authors: Y.Cho,J.H.Lee
Key ref: B.I.Khayrutdinov et al. (2009). Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors. Protein Sci, 18, 2252-2264. PubMed id: 19722278
Date:
06-Jul-09     Release date:   13-Oct-09    
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 Headers
 References

Protein chain
Q8R4E9  (CDT1_MOUSE) -  DNA replication factor Cdt1 from Mus musculus
Seq:
Struc: 		 
Seq:
Struc: 		557 a.a.
106 a.a.
Key:    Secondary structure  CATH domain

 

 
Protein Sci 18:2252-2264 (2009)
PubMed id: 19722278  
 
 
Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors.
B.I.Khayrutdinov, W.J.Bae, Y.M.Yun, J.H.Lee, T.Tsuyama, J.J.Kim, E.Hwang, K.S.Ryu, H.K.Cheong, C.Cheong, J.S.Ko, T.Enomoto, P.A.Karplus, P.Güntert, S.Tada, Y.H.Jeon, Y.Cho.
 
  ABSTRACT  
 
In eukaryotic replication licensing, Cdt1 plays a key role by recruiting the MCM2-7 complex onto the origin of chromosome. The C-terminal domain of mouse Cdt1 (mCdt1C), the most conserved region in Cdt1, is essential for licensing and directly interacts with the MCM2-7 complex. We have determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and solution NMR spectroscopy, respectively. While the N-terminal 31 residues of mCdt1CL form a flexible loop with a short helix near the middle, the rest of mCdt1C folds into a winged helix structure. Together with the middle domain of mouse Cdt1 (mCdt1M, residues 172-368), this study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain. The winged helix fold is also conserved in other licensing factors including archaeal ORC and Cdc6, which supports an idea that these replication initiators may have evolved from a common ancestor. Based on the structure of mCdt1C, in conjunction with the biochemical analysis, we propose a binding site for the MCM complex within the mCdt1C.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
  21278447 L.N.Truong, and X.Wu (2011).
Prevention of DNA re-replication in eukaryotic cells.
  J Mol Cell Biol, 3, 13-22.  
20335175 J.Jee, T.Mizuno, K.Kamada, H.Tochio, Y.Chiba, K.Yanagi, G.Yasuda, H.Hiroaki, F.Hanaoka, and M.Shirakawa (2010).
Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins.
  J Biol Chem, 285, 15931-15940.
PDB code: 2rqq
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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