PDBsum entry 3a1v

Transport protein

PDB id

3a1v

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Contents

			Protein chains

					252 a.a. *

			Ligands

					SO4 ×2


					MPD ×2


					EPE


					MRD

			Waters ×130

* Residue conservation analysis

PDB id:

3a1v

Links
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Name:

Transport protein

Title:

Crystal structue of the cytosolic domain of t. Maritima feob iron iransporter in apo form

Structure:

Iron(ii) transport protein b. Chain: a, b. Fragment: unp residues 17-269. Engineered: yes

Source:

Thermotoga maritima. Organism_taxid: 2336. Gene: tm0051. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.40Å

R-factor:

0.205

R-free:

0.242

Authors:

M.Hattori,R.Ishitani,O.Nureki

Key ref:

M.Hattori et al. (2009). Structural basis of novel interactions between the small-GTPase and GDI-like domains in prokaryotic FeoB iron transporter. Structure, 17, 1345-1355. PubMed id: 19733088

Date:

22-Apr-09

Release date:

22-Sep-09

PROCHECK

	Headers

	References

Protein chains

Q9WXQ8 (Q9WXQ8_THEMA) - Ferrous iron transport protein B from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

Seq: Struc:

Seq: Struc:					669 a.a. 252 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

	Structure 17:1345-1355 (2009)
PubMed id: 19733088

Structural basis of novel interactions between the small-GTPase and GDI-like domains in prokaryotic FeoB iron transporter.
M.Hattori, Y.Jin, H.Nishimasu, Y.Tanaka, M.Mochizuki, T.Uchiumi, R.Ishitani, K.Ito, O.Nureki.

ABSTRACT

The FeoB family proteins are widely distributed prokaryotic membrane proteins involved in Fe(2+) uptake. FeoB consists of N-terminal cytosolic and C-terminal transmembrane domains. The N-terminal region of the cytosolic domain is homologous to small GTPase (G) proteins and is considered to regulate Fe(2+) uptake. The spacer region connecting the G and TM domains reportedly functions as a GDP dissociation inhibitor (GDI)-like domain that stabilizes the GDP-binding state. However, the function of the G and GDI-like domains in iron uptake remains unclear. Here, we report the structural and functional analyses of the FeoB cytosolic domain from Thermotoga maritima. The structure-based mutational analysis indicated that the interaction between the G and GDI-like domains is important for both the GDI and Fe(2+) uptake activities. On the basis of these results, we propose a regulatory mechanism of Fe(2+) uptake.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20123128

K.W.Hung, Y.W.Chang, E.T.Eng, J.H.Chen, Y.C.Chen, Y.J.Sun, C.D.Hsiao, G.Dong, K.A.Spasov, V.M.Unger, and T.H.Huang (2010).
Structural fold, conservation and Fe(II) binding of the intracellular domain of prokaryote FeoB.

J Struct Biol, 170, 501-512.

PDB codes:

2wia 2wib 2wic 3k53

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.