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PDBsum entry 3njp

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protein ligands Protein-protein interface(s) links
Transferase PDB id
3njp
Jmol
Contents
Protein chains
614 a.a. *
47 a.a. *
Ligands
NAG ×12
2PE ×5
Waters ×24
* Residue conservation analysis
PDB id:
3njp
Name: Transferase
Title: The extracellular and transmembrane domain interfaces in epi growth factor receptor signaling
Structure: Epidermal growth factor receptor. Chain: a, b. Synonym: receptor tyrosine-protein kinase erbb-1, proto-onc erbb-1. Engineered: yes. Epidermal growth factor. Chain: c, d. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: egfr, erbb1. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_organ: ovary. Gene: egf. Expression_system_organ: ovary
Resolution:
3.30Å     R-factor:   0.265     R-free:   0.298
Authors: C.Lu,L.-Z.Mi,M.J.Grey,J.Zhu,E.Graef,S.Yokoyama,T.A.Springer
Key ref: C.Lu et al. (2010). Structural evidence for loose linkage between ligand binding and kinase activation in the epidermal growth factor receptor. Mol Cell Biol, 30, 5432-5443. PubMed id: 20837704 DOI: 10.1128/MCB.00742-10
Date:
17-Jun-10     Release date:   13-Oct-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00533  (EGFR_HUMAN) -  Epidermal growth factor receptor
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1210 a.a.
614 a.a.*
Protein chains
Pfam   ArchSchema ?
P01133  (EGF_HUMAN) -  Pro-epidermal growth factor
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1207 a.a.
47 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.2.7.10.1  - Receptor protein-tyrosine kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP
+
[protein]-L-tyrosine
Bound ligand (Het Group name = NAG)
matches with 47.00% similarity
= ADP
+ [protein]-L-tyrosine phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   1 term 
  Biological process     transmembrane receptor protein tyrosine kinase signaling pathway   2 terms 
  Biochemical function     ATP binding     2 terms  

 

 
    reference    
 
 
DOI no: 10.1128/MCB.00742-10 Mol Cell Biol 30:5432-5443 (2010)
PubMed id: 20837704  
 
 
Structural evidence for loose linkage between ligand binding and kinase activation in the epidermal growth factor receptor.
C.Lu, L.Z.Mi, M.J.Grey, J.Zhu, E.Graef, S.Yokoyama, T.A.Springer.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22218287 N.J.Bessman, and M.A.Lemmon (2012).
Finding the missing links in EGFR.
  Nat Struct Mol Biol, 19, 1-3.  
21822280 L.Z.Mi, C.Lu, Z.Li, N.Nishida, T.Walz, and T.A.Springer (2011).
Simultaneous visualization of the extracellular and cytoplasmic domains of the epidermal growth factor receptor.
  Nat Struct Mol Biol, 18, 984-989.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.