PDBsum entry: 3iv8

Hydrolase

PDB id: 3iv8

Contents

Protein chains

379 a.a. *

356 a.a. *

Ligands

F6P

SO4 ×3

Metals

_NI ×4

Waters ×332

* Residue conservation analysis

PDB id:

3iv8

Name:

Hydrolase

Title:

N-acetylglucosamine-6-phosphate deacetylase from vibrio chol complexed with fructose 6-phosphate

Structure:

N-acetylglucosamine-6-phosphate deacetylase. Chain: a, b, c, d. Synonym: glcnac 6-p deacetylase. Engineered: yes

Source:

Vibrio cholerae. Organism_taxid: 666. Strain: o1 biovar el tor str. N16961. Gene: naga, vc_0994. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.53Å R-factor: 0.186 R-free: 0.253

Authors:

J.Osipiuk,N.Maltseva,J.Stam,W.F.Anderson,A.Joachimiak,Center Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

J.Osipiuk et al. X-Ray crystal structure of n-Acetylglucosamine-6-Phos deacetylase from vibrio cholerae complexed with fruct 6-Phosphate.. To be published,

Date:

31-Aug-09 Release date: 08-Sep-09

Protein chains

O32445  (NAGA_VIBCH) -  N-acetylglucosamine-6-phosphate deacetylase

Seq: 378 a.a.

Struc: 379 a.a.

Protein chain

O32445  (NAGA_VIBCH) -  N-acetylglucosamine-6-phosphate deacetylase

Seq: 378 a.a.

Struc: 356 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.5.1.25 - N-acetylglucosamine-6-phosphate deacetylase.
• Pathway: UDP-N-acetylglucosamine Biosynthesis
• Reaction: N-acetyl-D-glucosamine 6-phosphate + H₂O = D-glucosamine 6-phosphate + acetate

N-acetyl-D-glucosamine 6-phosphate + H(2)O = D-glucosamine 6-phosphate (Bound ligand (Het Group name = F6P) matches with 88.24% similarity) + acetate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biological process: carbohydrate metabolic process (2 terms)
• Biochemical function: hydrolase activity (3 terms)