 |
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
|
|
|
|
|
|
|
|
|
Transferase, RNA binding protein
|
PDB id
|
|
|
|
3ief
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
PDB id:
|
 |
|
 |
| Name: |
 |
Transferase, RNA binding protein
|
 |
|
Title:
|
 |
Crystal structure of tRNA guanine-n1-methyltransferase from bartonella henselae using mpcs.
|
|
Structure:
|
 |
tRNA (guanine-n(1)-)-methyltransferase. Chain: a, b. Synonym: m1g-methyltransferase, tRNA [gm37] methyltransferase. Engineered: yes
|
|
Source:
|
 |
Bartonella henselae. Rochalimaea henselae. Organism_taxid: 38323. Strain: houston-1. Gene: trmd, bh15820. Expressed in: escherichia coli. Expression_system_taxid: 562.
|
|
Resolution:
|
 |
|
2.50Å
|
R-factor:
|
0.196
|
R-free:
|
0.225
|
|
|
Authors:
|
 |
T.E.Edwards,G.Stahl,C.J.Gerdts,B.L.Staker,Accelerated Technologies Center For Gene To 3d Structure (Atcg3d), Seattle Structural Genomics Center For Infectious Disease (Ssgcid)
|
|
Key ref:
|
 |
T.E.Edwards
et al.
Crystal structure of tRNA guanine-N1-Methyltransferase from bartonella henselae using mpcs.
To be published,
|
 |
|
Date:
|
 |
|
22-Jul-09
|
Release date:
|
11-Aug-09
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
Headers
|
 |
|
|
References
|
|
|
|
|
|
|
Q6G1R9
(TRMD_BARHE) -
tRNA (guanine-N(1)-)-methyltransferase
|
|
|
|
Seq: Struc:
|
 |
 |
 |
232 a.a.
224 a.a.
|
|
|
|
|
|
|
 |
 |
|
|
Key: |
 |
PfamA domain |
 |
 |
 |
Secondary structure |
 |
 |
CATH domain |
 |
|
|
|
|
 |
|
|
 |
 |
 |
 |
Enzyme class:
|
 |
E.C.2.1.1.228
- tRNA (guanine(37)-N(1))-methyltransferase.
|
|
 |
 |
 |
 |
 |
Reaction:
|
 |
S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
|
 |
 |
 |
 |
 |
S-adenosyl-L-methionine
|
+
|
guanine(37) in tRNA
|
=
|
S-adenosyl-L-homocysteine
|
+
|
N(1)-methylguanine(37) in tRNA
|
|
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
|
|
 |
 |
|
 |
|
 |
|
|
Gene Ontology (GO) functional annotation
|
|
|
|
 |
 |
 |
|
 |
 |
 |
 |
|
 |
|
Cellular component
|
cytoplasm
|
1 term
|
 |
|
Biological process
|
methylation
|
2 terms
|
 |
|
Biochemical function
|
transferase activity
|
4 terms
|
 |
|
|
 |
 |
 |
 |
 |
 |
|