PDBsum entry: 3cuy

Transferase

PDB id

3cuy

Contents

			Protein chain

					364 a.a. *

			Waters ×435

* Residue conservation analysis

PDB id:

3cuy

Links
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Name:

Transferase

Title:

Crystal structure of gumk mutant d157a

Structure:

Glucuronosyltransferase gumk. Chain: a. Engineered: yes. Mutation: yes

Source:

Xanthomonas campestris pv. Campestris. Gene: gumk. Expressed in: escherichia coli.

Resolution:

2.30Å

R-factor:

0.184

R-free:

0.225

Authors:

M.Barreras

Date:

17-Apr-08

Release date:

01-Jul-08

PROCHECK

	Headers

	References

Protein chain

Q8GCH2 (Q8GCH2_XANCP) - UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase

Seq: Struc:					400 a.a. 364 a.a.*

Key:

PfamA domain

Secondary structure

* PDB and UniProt seqs differ at 3 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.2.4.1.264 - D-man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1- diphospho-ditrans,octacis-undecaprenol = UDP + D-GlcA-beta-(1->2)-D-Man- alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis- undecaprenol

UDP-glucuronate	+	D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1- diphospho-ditrans,octacis-undecaprenol	=	UDP	+	D-GlcA-beta-(1->2)-D-Man- alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis- undecaprenol

Molecule diagrams generated from .mol files obtained from the KEGG ftp site



		Gene Ontology (GO) functional annotation

Cellular component	membrane	2 terms
Biological process	carbohydrate metabolic process	2 terms
Biochemical function	transferase activity	3 terms