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PDBsum entry 352d

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dna_rna metals links
DNA PDB id
352d
Jmol
Contents
DNA/RNA
Metals
_CA ×9
_NA ×14
Waters ×560
PDB id:
352d
Name: DNA
Title: The crystal structure of a parallel-stranded parallel- stranded guanine tetraplex at 0.95 angstrom resolution
Structure: DNA (5'-d( Tp Gp Gp Gp Gp T)-3'). Chain: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p. Engineered: yes
Source: Synthetic: yes
Biol. unit: Octamer (from PQS)
Resolution:
0.95Å     R-factor:   0.152    
Authors: K.Phillips,Z.Dauter,A.I.H.Murchie,D.M.J.Lilley,B.Luisi
Key ref:
K.Phillips et al. (1997). The crystal structure of a parallel-stranded guanine tetraplex at 0.95 A resolution. J Mol Biol, 273, 171-182. PubMed id: 9367755 DOI: 10.1006/jmbi.1997.1292
Date:
04-Sep-97     Release date:   10-Nov-97    
 Headers
 References

 

 
DOI no: 10.1006/jmbi.1997.1292 J Mol Biol 273:171-182 (1997)
PubMed id: 9367755  
 
 
The crystal structure of a parallel-stranded guanine tetraplex at 0.95 A resolution.
K.Phillips, Z.Dauter, A.I.Murchie, D.M.Lilley, B.Luisi.
 
  ABSTRACT  
 
In both DNA and RNA, stretches of guanine bases can form stable four-stranded helices in the presence of sodium or potassium ions. Sequences with a propensity to form guanine tetraplexes have been found in chromosomal telomers, immunoglobulin switch regions, and recombination sites. We report the crystal structure at 0.95 A resolution of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) in the presence of sodium ions. The four strands form a right-handed helix that is stabilized by hydrogen-bonding tetrads of co-planar guanine bases. Well-resolved sodium ions are found between and, at defined points, within tetrad planes and are coordinated with the guanine O6 groups. Nine calcium ions have been identified, each with a well-defined hepta-coordinate hydration shell. Hydrogen-bonding water patterns are observed within the tetraplex's helical grooves and clustered about the phosphate groups. Water molecules in the groove may form a hydrogen bond with the O4', and may affect the stacking behavior of guanine. Two distinct stacking arrangements are noted for the guanine tetrads. The thymine bases do not contribute to the four-stranded conformation, but instead stack to stabilize the crystal lattice. We present evidence that the sugar conformation is strained and propose that this originates from forces that optimize guanine base stacking. Discrete conformational disorder is observed at several places in the phosphodiester backbone, which results from a simple crankshaft rotation that requires no net change in the sugar conformation.
 
  Selected figure(s)  
 
Figure 5.
Figure 5. Stereoscopic view of the electron density revealing a representative calcium ion.
Figure 6.
Figure 6. (a) Stereoscopic view of the electron density of a representative groove, showing the hydration spine. All 16 crystallographically independent grooves have similar hydration spines. For clarity, the electron density is shown only for the water molecules (small red spheres). Hydrogen bonds are indicated by broken lines. (b) A stereoscopic view of hydration within the grooves: the distribution of water molecules around the averaged guanine:guanine base pair. (c) A stereoscopic view of the distribution of water molecules around the 28 observed thymine bases.
 
  The above figures are reprinted by permission from Elsevier: J Mol Biol (1997, 273, 171-182) copyright 1997.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
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Beyond nucleic acid base pairs: from triads to heptads.
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G-quadruplex DNA structures--variations on a theme.
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PDB code: 1be5
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.