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PDBsum entry 2zrs

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protein metals Protein-protein interface(s) links
Apoptosis PDB id
2zrs
Jmol
Contents
Protein chain
(+ 2 more) 165 a.a. *
Metals
_CA ×25
* Residue conservation analysis
PDB id:
2zrs
Name: Apoptosis
Title: Crystal structure of ca2+-bound form of des3-23alg-2
Structure: Programmed cell death protein 6. Chain: a, b, c, d, e, f, g, h. Fragment: unp residues 24-191. Synonym: apoptosis-linked gene 2 protein, calcium-binding protein alg-2. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: pdcd6, alg2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
3.10Å     R-factor:   0.228     R-free:   0.280
Authors: H.Suzuki,M.Kawasaki,T.Kakiuchi,H.Shibata,S.Wakatsuki,M.Maki
Key ref: H.Suzuki et al. (2008). Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2. Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 974-977. PubMed id: 18997320
Date:
01-Sep-08     Release date:   04-Nov-08    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
O75340  (PDCD6_HUMAN) -  Programmed cell death protein 6
Seq:
Struc:
191 a.a.
165 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     calcium ion binding     1 term  

 

 
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:974-977 (2008)
PubMed id: 18997320  
 
 
Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2.
H.Suzuki, M.Kawasaki, T.Kakiuchi, H.Shibata, S.Wakatsuki, M.Maki.
 
  ABSTRACT  
 
ALG-2 (apoptosis-linked gene 2) is an apoptosis-linked calcium-binding protein with five EF-hand motifs in the C-terminal region. N-terminally truncated ALG-2 (des3-23ALG-2) was crystallized by the vapour-diffusion method in buffer consisting of either 50 mM MES pH 6.5, 12.5%(v/v) 2-propanol and 150 mM calcium acetate or 100 mM MES pH 6.0, 15%(v/v) ethanol and 200 mM zinc acetate. Crystals of the Ca(2+)-bound form belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 54.8, b = 154.4, c = 237.7 A, alpha = beta = gamma = 90 degrees , and diffracted to 3.1 A resolution. Crystals of the Zn(2+)-bound form belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.8, b = 147.5, c = 230.7 A, alpha = beta = gamma = 90 degrees , and diffracted to 3.3 A resolution. The structures of the Ca(2+)-bound form and the Zn(2+)-bound form were solved by the molecular-replacement method. Although both crystals contained eight ALG-2 molecules per asymmetric unit, the metal-ion locations and octameric arrangements were found to be significantly different.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
  20691033 T.Inuzuka, H.Suzuki, M.Kawasaki, H.Shibata, S.Wakatsuki, and M.Maki (2010).
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
  BMC Struct Biol, 10, 25.
PDB codes: 3aaj 3aak
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