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PDBsum entry 2ze9

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protein ligands links
Hydrolase PDB id
2ze9
Jmol
Contents
Protein chain
504 a.a. *
Ligands
MES
PD7
Waters ×365
* Residue conservation analysis
PDB id:
2ze9
Name: Hydrolase
Title: Crystal structure of h168a mutant of phospholipase d from streptomyces antibioticus, as a complex with phosphatidylcholine
Structure: Phospholipase d. Chain: a. Synonym: choline phosphatase. Engineered: yes. Mutation: yes
Source: Streptomyces antibioticus. Organism_taxid: 1890. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.30Å     R-factor:   0.174     R-free:   0.251
Authors: A.Suzuki,H.Toda,Y.Iwasaki,T.Yamane,T.Yamane
Key ref: A.Suzuki et al. Crystal structure of phospholipase d from streptomyces antibioticus. To be published, .
Date:
06-Dec-07     Release date:   25-Dec-07    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q53728  (PLD_STRAT) -  Phospholipase D
Seq:
Struc:
 
Seq:
Struc:
556 a.a.
504 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.1.4.4  - Phospholipase D.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: A phosphatidylcholine + H2O = choline + a phosphatidate
phosphatidylcholine
Bound ligand (Het Group name = PD7)
matches with 59.00% similarity
+ H(2)O
=
choline
Bound ligand (Het Group name = MES)
matches with 46.00% similarity
+ phosphatidate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     catalytic activity     4 terms