PDBsum entry: 2ze9

Hydrolase

PDB id: 2ze9

Contents

Protein chain

504 a.a. *

Ligands

MES

PD7

Waters ×365

* Residue conservation analysis

PDB id:

2ze9

Name:

Hydrolase

Title:

Crystal structure of h168a mutant of phospholipase d from streptomyces antibioticus, as a complex with phosphatidylcholine

Structure:

Phospholipase d. Chain: a. Synonym: choline phosphatase. Engineered: yes. Mutation: yes

Source:

Streptomyces antibioticus. Organism_taxid: 1890. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.30Å R-factor: 0.174 R-free: 0.251

Authors:

A.Suzuki,H.Toda,Y.Iwasaki,T.Yamane,T.Yamane

Key ref:

A.Suzuki et al. Crystal structure of phospholipase d from streptomyces antibioticus. To be published,

Date:

06-Dec-07 Release date: 25-Dec-07

Protein chain

Q53728  (PLD_STRAT) -  Phospholipase D

Seq: 556 a.a.

Struc: 504 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.4.4 - Phospholipase D.
• Reaction: A phosphatidylcholine + H₂O = choline + a phosphatidate

phosphatidylcholine (Bound ligand (Het Group name = PD7) matches with 59.00% similarity) + H(2)O = choline (Bound ligand (Het Group name = MES) matches with 46.00% similarity) + phosphatidate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: extracellular region (1 term)
• Biological process: metabolic process (2 terms)
• Biochemical function: catalytic activity (4 terms)