PDBsum entry 2zcw

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protein ligands links
Transcription PDB id
Protein chain
194 a.a. *
MPD ×3
Waters ×141
* Residue conservation analysis
PDB id:
Name: Transcription
Title: Crystal structure of ttha1359, a transcriptional regulator, crp/fnr family from thermus thermophilus hb8
Structure: Transcriptional regulator, fnr/crp family. Chain: a. Synonym: ttha1359. Engineered: yes
Source: Thermus thermophilus. Organism_taxid: 274. Strain: hb8. Expressed in: escherichia coli. Expression_system_taxid: 562.
1.50Å     R-factor:   0.221     R-free:   0.232
Authors: Y.Agari,S.Yokoyama,S.Kuramitsu,Riken Structural Genomics/proteomics Initiative (Rsgi)
Key ref: Y.Agari et al. (2008). Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator. Mol Microbiol, 70, 60-75. PubMed id: 18699868
13-Nov-07     Release date:   04-Dec-07    
Supersedes: 2coh
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
Q5SIL0  (Q5SIL0_THET8) -  Transcriptional regulator, FNR/CRP family
202 a.a.
194 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   1 term 
  Biological process     transcription, DNA-dependent   2 terms 
  Biochemical function     DNA binding     2 terms  


Mol Microbiol 70:60-75 (2008)
PubMed id: 18699868  
Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator.
Y.Agari, A.Kashihara, S.Yokoyama, S.Kuramitsu, A.Shinkai.
Thermus thermophilus SdrP is one of four cyclic AMP receptor protein (CRP)/fumarate and nitrate reduction regulator (FNR) family proteins from the extremely thermophilic bacterium T. thermophilus HB8. Expression of sdrP mRNA increased in the stationary phase during cultivation at 70 degrees C. Although the sdrP gene was non-essential, an sdrP-deficient strain showed growth defects, particularly when grown in a synthetic medium, and increased sensitivity to disulphide stress. The expression of several genes was altered in the sdrP disruptant. Among them, we found eight SdrP-dependent promoters using in vitro transcription assays. A predicted SdrP binding site similar to that recognized by Escherichia coli CRP was found upstream of each SdrP-dependent promoter. In the wild-type strain, expression of these eight genes tended to increase upon entry into the stationary phase. Transcriptional activation in vitro was independent of any added effector molecule. The hypothesis that apo-SdrP is the active form of the protein was supported by the observation that the three-dimensional structure of apo-SdrP is similar to that of the DNA-binding form of E. coli CRP. Based on the properties of the SdrP-regulated genes found in this study, it is speculated that SdrP is involved in nutrient and energy supply, redox control, and polyadenylation of mRNA.

Literature references that cite this PDB file's key reference

  PubMed id Reference
20395270 K.Sakamoto, Y.Agari, K.Agari, S.Kuramitsu, and A.Shinkai (2010).
Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8.
  Microbiology, 156, 1993-2005.
PDB code: 3aai
21054499 Y.Agari, S.Kuramitsu, and A.Shinkai (2010).
Identification of novel genes regulated by the oxidative stress-responsive transcriptional activator SdrP in Thermus thermophilus HB8.
  FEMS Microbiol Lett, 313, 127-134.  
19193643 D.T.Gallagher, N.Smith, S.K.Kim, H.Robinson, and P.T.Reddy (2009).
Profound Asymmetry in the Structure of the cAMP-free cAMP Receptor Protein (CRP) from Mycobacterium tuberculosis.
  J Biol Chem, 284, 8228-8232.  
19955406 S.Mesa, L.Reutimann, H.M.Fischer, and H.Hennecke (2009).
Posttranslational control of transcription factor FixK2, a key regulator for the Bradyrhizobium japonicum-soybean symbiosis.
  Proc Natl Acad Sci U S A, 106, 21860-21865.  
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