spacer
spacer

PDBsum entry 2ypw

Go to PDB code: 
protein Protein-protein interface(s) links
Membrane protein PDB id
2ypw
Jmol
Contents
Protein chains
(+ 8 more) 112 a.a.
PDB id:
2ypw
Name: Membrane protein
Title: Atomic model for the n-terminus of trao fitted in the full-l structure of the bacterial pkm101 type iv secretion system complex
Structure: Trao. Chain: a, b, c, d, e, f, g, h, i, j, k, l, m, n. Fragment: n-terminal domain, residues 24-135. Engineered: yes
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Authors: A.Rivera-Calzada,R.Fronzes,C.G.Savva,V.Chandran,P.W.Lian,T.L E.Pardon,J.Steyaert,H.Remaut,G.Waksman,E.V.Orlova
Key ref: A.Rivera-Calzada et al. (2013). Structure of a bacterial type IV secretion core complex at subnanometre resolution. EMBO J, 32, 1195-1204. PubMed id: 23511972 DOI: 10.1038/emboj.2013.58
Date:
02-Nov-12     Release date:   03-Apr-13    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q46704  (Q46704_ECOLX) -  TraO protein
Seq:
Struc:
294 a.a.
112 a.a.
Key:    PfamA domain  Secondary structure

 

 
DOI no: 10.1038/emboj.2013.58 EMBO J 32:1195-1204 (2013)
PubMed id: 23511972  
 
 
Structure of a bacterial type IV secretion core complex at subnanometre resolution.
A.Rivera-Calzada, R.Fronzes, C.G.Savva, V.Chandran, P.W.Lian, T.Laeremans, E.Pardon, J.Steyaert, H.Remaut, G.Waksman, E.V.Orlova.
 
  ABSTRACT  
 
Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.