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PDBsum entry 2yfh

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protein Protein-protein interface(s) links
Oxidoreductase PDB id
2yfh
Jmol
Contents
Protein chains
(+ 0 more) 444 a.a.
Waters ×313
PDB id:
2yfh
Name: Oxidoreductase
Title: Structure of a chimeric glutamate dehydrogenase
Structure: Glutamate dehydrogenase, NAD-specific glutamate dehydrogenase, glutamate dehydrogenase. Chain: a, b, c, d, e, f. Fragment: residues 1-200,202-405,407-450. Engineered: yes
Source: Clostridium symbiosum, escherichia col clostridium symbiosum. Organism_taxid: 1512, 562, 1512. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: delta gdha.
Resolution:
2.70Å     R-factor:   0.218     R-free:   0.282
Authors: T.Oliveira,M.A.Sharkey,M.Hamza,P.C.Engel,A.R.Khan
Key ref: T.Oliveira et al. Structural determinants of domain flexibility in bact glutamate dehydrogenases. To be published, .
Date:
05-Apr-11     Release date:   04-Apr-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P24295  (DHE2_CLOSY) -  NAD-specific glutamate dehydrogenase
Seq:
Struc:
450 a.a.
444 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 100 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.4.1.2  - Glutamate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH
L-glutamate
+ H(2)O
+ NAD(+)
= 2-oxoglutarate
+ NH(3)
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   3 terms 
  Biochemical function     oxidoreductase activity     3 terms