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PDBsum entry 2y9x

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protein ligands metals Protein-protein interface(s) links
Oxidoreductase PDB id
2y9x
Jmol
Contents
Protein chains
391 a.a.
136 a.a.
Ligands
0TR ×4
Metals
_CU ×8
_HO ×4
Waters ×25
PDB id:
2y9x
Name: Oxidoreductase
Title: Crystal structure of ppo3, a tyrosinase from agaricus bispor deoxy-form that contains additional unknown lectin-like sub with inhibitor tropolone
Structure: Polyphenol oxidase. Chain: a, b, c, d. Fragment: residues 2-392. Synonym: tyrosinase. Other_details: thioether bond between his85 and cys83. Lectin-like fold protein. Chain: e, f, g, h
Source: Agaricus bisporus. Common mushroom. Organism_taxid: 5341. Other_details: commercial. Other_details: commercial
Resolution:
2.78Å     R-factor:   0.238     R-free:   0.289
Authors: W.T.Ismaya,H.J.Rozeboom,A.Weijn,J.J.Mes,F.Fusetti,H.J.Wicher B.W.Dijkstra
Key ref: W.T.Ismaya et al. (2011). Crystal structure of Agaricus bisporus mushroom tyrosinase: identity of the tetramer subunits and interaction with tropolone. Biochemistry, 50, 5477-5486. PubMed id: 21598903
Date:
17-Feb-11     Release date:   06-Jul-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
C7FF04  (PPO3_AGABI) -  Polyphenol oxidase 3
Seq:
Struc:
 
Seq:
Struc:
576 a.a.
391 a.a.
Protein chains
Pfam   ArchSchema ?
G1K3P4  (G1K3P4_AGABI) -  Lectin-like fold protein
Seq:
Struc:
150 a.a.
136 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D: E.C.1.14.18.1  - Tyrosinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Melanin Biosynthesis
      Reaction:
1. 2 L-dopa + O2 = 2 dopaquinone + 2 H2O
2. L-tyrosine + O2 = dopaquinone + H2O
2 × L-dopa
Bound ligand (Het Group name = 0TR)
matches with 69.23% similarity
+ O(2)
= 2 × dopaquinone
+ 2 × H(2)O
L-tyrosine
+ O(2)
= dopaquinone
+ H(2)O
      Cofactor: Copper
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   1 term 
  Biochemical function     oxidoreductase activity     1 term  

 

 
    reference    
 
 
Biochemistry 50:5477-5486 (2011)
PubMed id: 21598903  
 
 
Crystal structure of Agaricus bisporus mushroom tyrosinase: identity of the tetramer subunits and interaction with tropolone.
W.T.Ismaya, H.J.Rozeboom, A.Weijn, J.J.Mes, F.Fusetti, H.J.Wichers, B.W.Dijkstra.
 
  ABSTRACT  
 
No abstract given.