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PDBsum entry 2y6t

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protein ligands Protein-protein interface(s) links
Hydrolase/inhibitor PDB id
2y6t
Jmol
Contents
Protein chains
240 a.a.
141 a.a.
Ligands
SO4 ×4
Waters ×33
PDB id:
2y6t
Name: Hydrolase/inhibitor
Title: Molecular recognition of chymotrypsin by the serine protease inhibitor ecotin from yersinia pestis
Structure: Chymotrypsinogen a. Chain: a, b, c, d. Synonym: chymotrypsin. Engineered: yes. Ecotin. Chain: e, f, g, h. Engineered: yes
Source: Bos taurus. Cattle. Organism_taxid: 9913. Other_details: activated bovine chymotrypsin was purchased sigma-aldrich. Yersinia pseudotuberculosis. Organism_taxid: 502800. Strain: ypiii. Expressed in: escherichia coli.
Resolution:
2.74Å     R-factor:   0.247     R-free:   0.314
Authors: E.A.Clark,N.Walker,D.C.Ford,I.A.Cooper,P.C.F.Oyston,K.R.Acha
Key ref: E.A.Clark et al. (2011). Molecular recognition of chymotrypsin by the serine protease inhibitor ecotin from Yersinia pestis. J Biol Chem, 286, 24015-24022. PubMed id: 21531711 DOI: 10.1074/jbc.M111.225730
Date:
26-Jan-11     Release date:   20-Apr-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00766  (CTRA_BOVIN) -  Chymotrypsinogen A
Seq:
Struc:
245 a.a.
240 a.a.
Protein chains
Pfam   ArchSchema ?
B1JSA0  (ECOT_YERPY) -  Ecotin
Seq:
Struc:
169 a.a.
141 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D: E.C.3.4.21.1  - Chymotrypsin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   3 terms 
  Biological process     digestion   4 terms 
  Biochemical function     catalytic activity     8 terms  

 

 
DOI no: 10.1074/jbc.M111.225730 J Biol Chem 286:24015-24022 (2011)
PubMed id: 21531711  
 
 
Molecular recognition of chymotrypsin by the serine protease inhibitor ecotin from Yersinia pestis.
E.A.Clark, N.Walker, D.C.Ford, I.A.Cooper, P.C.Oyston, K.R.Acharya.
 
  ABSTRACT  
 
No abstract given.