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PDBsum entry 2y3a

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protein ligands Protein-protein interface(s) links
Transferase PDB id
2y3a
Jmol
Contents
Protein chains
976 a.a.
236 a.a.
Ligands
GD9
PDB id:
2y3a
Name: Transferase
Title: Crystal structure of p110beta in complex with icsh2 of p85be the drug gdc-0941
Structure: Phosphatidylinositol-4,5-bisphosphate 3-kinase ca subunit beta isoform. Chain: a. Synonym: pi3-kinase subunit beta, pi3k-beta, ptdins-3-kinas subunit beta, phosphatidylinositol-4,5-bisphosphate 3-kina kda catalytic subunit beta, ptdins-3-kinase subunit p110-b pik3ca. Engineered: yes. Phosphatidylinositol 3-kinase regulatory subunit
Source: Mus musculus. Mouse. Organism_taxid: 10090. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf-9.
Resolution:
3.30Å     R-factor:   0.237     R-free:   0.296
Authors: X.Zhang,O.Vadas,O.Perisic,R.L.Williams
Key ref: X.Zhang et al. (2011). Structure of lipid kinase p110β/p85β elucidates an unusual SH2-domain-mediated inhibitory mechanism. Mol Cell, 41, 567-578. PubMed id: 21362552
Date:
20-Dec-10     Release date:   16-Mar-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q8BTI9  (PK3CB_MOUSE) -  Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1064 a.a.
976 a.a.
Protein chain
Pfam   ArchSchema ?
O08908  (P85B_MOUSE) -  Phosphatidylinositol 3-kinase regulatory subunit beta
Seq:
Struc:
 
Seq:
Struc:
722 a.a.
236 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain A: E.C.2.7.1.153  - Phosphatidylinositol-4,5-bisphosphate 3-kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
1-Phosphatidyl-myo-inositol Metabolism
      Reaction: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP
+ 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
= ADP
+ 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     plasma membrane   4 terms 
  Biological process     phosphatidylinositol-3-phosphate biosynthetic process   12 terms 
  Biochemical function     nucleotide binding     12 terms  

 

 
    reference    
 
 
Mol Cell 41:567-578 (2011)
PubMed id: 21362552  
 
 
Structure of lipid kinase p110β/p85β elucidates an unusual SH2-domain-mediated inhibitory mechanism.
X.Zhang, O.Vadas, O.Perisic, K.E.Anderson, J.Clark, P.T.Hawkins, L.R.Stephens, R.L.Williams.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22358332 B.Vanhaesebroeck, L.Stephens, and P.Hawkins (2012).
PI3K signalling: the path to discovery and understanding.
  Nat Rev Mol Cell Biol, 13, 195-203.  
21362546 P.K.Vogt (2011).
PI3K p110β: more tightly controlled or constitutively active?
  Mol Cell, 41, 499-501.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.