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PDBsum entry 2y0s

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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
2y0s
Jmol
Contents
Protein chains
831 a.a. *
1085 a.a. *
368 a.a. *
262 a.a. *
164 a.a. *
90 a.a. *
110 a.a. *
74 a.a. *
82 a.a. *
39 a.a. *
91 a.a. *
64 a.a. *
45 a.a. *
Ligands
F3S ×2
Metals
_MG ×2
_ZN ×14
* Residue conservation analysis
PDB id:
2y0s
Name: Transferase
Title: Crystal structure of sulfolobus shibatae RNA polymerase in p21 space group
Structure: DNA-directed RNA polymerase. Chain: a, w. DNA-directed RNA polymerase. Chain: b, r. DNA-directed RNA polymerase subunit a''. Chain: c, y. DNA-directed RNA polymerase subunit d. Chain: d, s. RNA polymerase subunit 4.
Source: Sulfolobus shibatae. Organism_taxid: 2286. Organism_taxid: 2286
Resolution:
3.80Å     R-factor:   0.302     R-free:   0.353
Authors: M.Wojtas,B.Peralta,M.Ondiviela,M.Mogni,S.D.Bell,N.G.A.Abresc
Key ref: M.Wojtas et al. (2011). Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit. Biochem Soc Trans, 39, 25-30. PubMed id: 21265742
Date:
07-Dec-10     Release date:   16-Feb-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
B8YB53  (B8YB53_SULSH) -  DNA-directed RNA polymerase
Seq:
Struc:
 
Seq:
Struc:
880 a.a.
831 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB55  (B8YB55_SULSH) -  DNA-directed RNA polymerase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1131 a.a.
1085 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB54  (B8YB54_SULSH) -  DNA-directed RNA polymerase subunit A''
Seq:
Struc:
395 a.a.
368 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB56  (B8YB56_SULSH) -  DNA-directed RNA polymerase subunit D
Seq:
Struc:
265 a.a.
262 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB57  (B8YB57_SULSH) -  RNA polymerase subunit 7
Seq:
Struc:
180 a.a.
164 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB58  (B8YB58_SULSH) -  RNA polymerase subunit 4
Seq:
Struc:
113 a.a.
90 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB59  (B8YB59_SULSH) -  RNA polymerase subunit 8
Seq:
Struc:
132 a.a.
110 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB60  (B8YB60_SULSH) -  DNA-directed RNA polymerase subunit H
Seq:
Struc:
84 a.a.
74 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB61  (B8YB61_SULSH) -  DNA-directed RNA polymerase subunit K
Seq:
Struc:
95 a.a.
82 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB65  (B8YB65_SULSH) -  RNA polymerase subunit 13
Seq:
Struc:
104 a.a.
39 a.a.*
Protein chains
Pfam   ArchSchema ?
B8YB62  (B8YB62_SULSH) -  DNA-directed RNA polymerase subunit L
Seq:
Struc:
92 a.a.
91 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB63  (B8YB63_SULSH) -  DNA-directed RNA polymerase subunit N
Seq:
Struc:
66 a.a.
64 a.a.
Protein chains
Pfam   ArchSchema ?
B8YB64  (B8YB64_SULSH) -  DNA-directed RNA polymerase subunit P
Seq:
Struc:
48 a.a.
45 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D, H, I, K, L, M, N, O, P, R, S, W, X, Y, Z: E.C.2.7.7.6  - DNA-directed Rna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate
+ RNA(n)
= diphosphate
+ RNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   1 term 
  Biological process     cellular metabolic process   2 terms 
  Biochemical function     catalytic activity     15 terms  

 

 
    reference    
 
 
Biochem Soc Trans 39:25-30 (2011)
PubMed id: 21265742  
 
 
Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit.
M.Wojtas, B.Peralta, M.Ondiviela, M.Mogni, S.D.Bell, N.G.Abrescia.
 
  ABSTRACT  
 
No abstract given.