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PDBsum entry 2xxb

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
2xxb
Jmol
Contents
Protein chains
310 a.a. *
Ligands
AMP ×2
Waters ×293
* Residue conservation analysis
PDB id:
2xxb
Name: Oxidoreductase
Title: Penta-mutant of thermus thermophilus lactate dehydrogenase, complex with amp
Structure: L-lactate dehydrogenase. Chain: a, b. Synonym: l-ldh. Engineered: yes. Mutation: yes
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.15Å     R-factor:   0.222     R-free:   0.288
Authors: F.Diop,N.Coquelle,F.M.D.Vellieux
Key ref: F.Diop et al. Lactate dehydrogenase from t. Thermophilus, Penta-Mut (complex with amp). To be published, .
Date:
09-Nov-10     Release date:   17-Nov-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q5SJA1  (LDH_THET8) -  L-lactate dehydrogenase
Seq:
Struc:
310 a.a.
310 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.1.1.27  - L-lactate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: (S)-lactate + NAD+ = pyruvate + NADH
(S)-lactate
+
NAD(+)
Bound ligand (Het Group name = AMP)
matches with 52.00% similarity
= pyruvate
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     oxidation-reduction process   4 terms 
  Biochemical function     catalytic activity     4 terms