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PDBsum entry 2xn2

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protein ligands links
Hydrolase PDB id
2xn2
Jmol
Contents
Protein chain
729 a.a.
Ligands
GLA
IMD
GOL ×25
Waters ×624
PDB id:
2xn2
Name: Hydrolase
Title: Structure of alpha-galactosidase from lactobacillus acidophi with galactose
Structure: Alpha-galactosidase. Chain: a. Engineered: yes
Source: Lactobacillus acidophilus ncfm. Organism_taxid: 272621. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.58Å     R-factor:   0.131     R-free:   0.155
Authors: F.Fredslund,M.Abou Hachem,R.J.Larsen,P.G.Sorensen,L.Lo Leggi B.Svensson
Key ref: F.Fredslund et al. (2011). Crystal structure of α-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding. J Mol Biol, 412, 466-480. PubMed id: 21827767 DOI: 10.1016/j.jmb.2011.07.057
Date:
30-Jul-10     Release date:   10-Aug-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q7WWP9  (Q7WWP9_LACAI) -  Alpha-galactosidase
Seq:
Struc:
 
Seq:
Struc:
732 a.a.
729 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.22  - Alpha-galactosidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Melibiose + H2O = galactose + glucose

+
=
+
      Cofactor: Mg(2+); NAD(+)
Mg(2+)
NAD(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   2 terms 
  Biochemical function     catalytic activity     6 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.jmb.2011.07.057 J Mol Biol 412:466-480 (2011)
PubMed id: 21827767  
 
 
Crystal structure of α-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding.
F.Fredslund, M.A.Hachem, R.J.Larsen, P.G.Sørensen, P.M.Coutinho, L.Lo Leggio, B.Svensson.
 
  ABSTRACT  
 
No abstract given.