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PDBsum entry 2x75

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protein ligands metals links
Lyase PDB id
2x75
Jmol
Contents
Protein chain
427 a.a. *
Ligands
OXL
AMP
P6G
Metals
_CL
Waters ×70
* Residue conservation analysis
PDB id:
2x75
Name: Lyase
Title: Staphylococcus aureus adenylosuccinate lyase
Structure: Adenylosuccinate lyase. Chain: a. Engineered: yes
Source: Staphylococcus aureus. Organism_taxid: 1280. Strain: 35556. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: american type culture collection
Resolution:
2.50Å     R-factor:   0.217     R-free:   0.284
Authors: P.K.Fyfe,A.Dawson,M.T.Hutchison,S.Cameron,W.N.Hunter
Key ref: P.K.Fyfe et al. (2010). Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery. Acta Crystallogr D Biol Crystallogr, 66, 881-888. PubMed id: 20693687
Date:
23-Feb-10     Release date:   09-Mar-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q7A0G9  (PUR8_STAAW) -  Adenylosuccinate lyase
Seq:
Struc:
431 a.a.
427 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.3.2.2  - Adenylosuccinate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Purine Biosynthesis (late stages)
      Reaction:
1. N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP
2. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
N(6)-(1,2-dicarboxyethyl)AMP
=
fumarate
Bound ligand (Het Group name = OXL)
matches with 40.00% similarity
+
AMP
Bound ligand (Het Group name = AMP)
corresponds exactly
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate
=
fumarate
Bound ligand (Het Group name = OXL)
matches with 40.00% similarity
+
5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
Bound ligand (Het Group name = AMP)
matches with 87.50% similarity
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     'de novo' AMP biosynthetic process   4 terms 
  Biochemical function     catalytic activity     4 terms  

 

 
    reference    
 
 
Acta Crystallogr D Biol Crystallogr 66:881-888 (2010)
PubMed id: 20693687  
 
 
Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery.
P.K.Fyfe, A.Dawson, M.T.Hutchison, S.Cameron, W.N.Hunter.
 
  ABSTRACT  
 
No abstract given.