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PDBsum entry 2x5h
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Viral protein
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PDB id
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2x5h
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PDB id:
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| Name: |
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Viral protein
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Title:
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Crystal structure of the orf131 l26m l51m double mutant from sulfolobus islandicus rudivirus 1
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Structure:
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Orf 131. Chain: a, b, c, d. Fragment: truncated version, residues 1-96. Synonym: uncharacterized protein 131, cag38830. Engineered: yes. Mutation: yes
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Source:
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Sulfolobus islandicus rudivirus 1. Organism_taxid: 282066. Variant: xx. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: bl21.
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Resolution:
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1.80Å
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R-factor:
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0.203
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R-free:
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0.257
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Authors:
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M.Oke,L.G.Carter,K.A.Johnson,H.Liu,S.A.Mcmahon,J.H.Naismith,M.F.White
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Key ref:
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M.Oke
et al.
(2010).
The Scottish Structural Proteomics Facility: targets, methods and outputs.
J Struct Funct Genomics,
11,
167-180.
PubMed id:
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Date:
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08-Feb-10
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Release date:
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21-Jul-10
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PROCHECK
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Headers
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References
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Q8QL44
(Y131_SIRV1) -
Uncharacterized protein 131 from Sulfolobus islandicus rod-shaped virus 1
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Seq:
Struc:
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131 a.a.
92 a.a.*
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Key: |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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J Struct Funct Genomics
11:167-180
(2010)
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PubMed id:
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The Scottish Structural Proteomics Facility: targets, methods and outputs.
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M.Oke,
L.G.Carter,
K.A.Johnson,
H.Liu,
S.A.McMahon,
X.Yan,
M.Kerou,
N.D.Weikart,
N.Kadi,
M.A.Sheikh,
S.Schmelz,
M.Dorward,
M.Zawadzki,
C.Cozens,
H.Falconer,
H.Powers,
I.M.Overton,
C.A.van Niekerk,
X.Peng,
P.Patel,
R.A.Garrett,
D.Prangishvili,
C.H.Botting,
P.J.Coote,
D.T.Dryden,
G.J.Barton,
U.Schwarz-Linek,
G.L.Challis,
G.L.Taylor,
M.F.White,
J.H.Naismith.
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ABSTRACT
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The Scottish Structural Proteomics Facility was funded to develop a laboratory
scale approach to high throughput structure determination. The effort was
successful in that over 40 structures were determined. These structures and the
methods harnessed to obtain them are reported here. This report reflects on the
value of automation but also on the continued requirement for a high degree of
scientific and technical expertise. The efficiency of the process poses
challenges to the current paradigm of structural analysis and publication. In
the 5 year period we published ten peer-reviewed papers reporting structural
data arising from the pipeline. Nevertheless, the number of structures solved
exceeded our ability to analyse and publish each new finding. By reporting the
experimental details and depositing the structures we hope to maximize the
impact of the project by allowing others to follow up the relevant biology.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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G.Hagelueken,
H.Huang,
K.Harlos,
B.R.Clarke,
C.Whitfield,
and
J.H.Naismith
(2012).
Crystallization, dehydration and experimental phasing of WbdD, a bifunctional kinase and methyltransferase from Escherichia coli O9a.
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Acta Crystallogr D Biol Crystallogr,
68,
1371-1379.
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PDB code:
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H.J.Kang,
and
E.N.Baker
(2011).
Intramolecular isopeptide bonds: protein crosslinks built for stress?
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Trends Biochem Sci,
36,
229-237.
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V.H.Tierrafría,
H.E.Ramos-Aboites,
G.Gosset,
and
F.Barona-Gómez
(2011).
Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.
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Microb Biotechnol,
4,
275-285.
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Y.S.Choong,
T.S.Lim,
A.L.Chew,
I.Aziah,
and
A.Ismail
(2011).
Structural and functional studies of a 50 kDa antigenic protein from Salmonella enterica serovar Typhi.
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J Mol Graph Model,
29,
834-842.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
