PDBsum entry: 2wt4

Hydrolase

PDB id: 2wt4

Contents

Protein chain

315 a.a. *

Ligands

ASP

Waters ×168

* Residue conservation analysis

PDB id:

2wt4

Name:

Hydrolase

Title:

Room temperature crystal structure of helicobacter pylori l- asparaginase at 1.8 a resolution

Structure:

L-asparaginase. Chain: a. Synonym: l-asnase. Engineered: yes

Source:

Helicobacter pylori. Organism_taxid: 210. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.80Å R-factor: 0.153 R-free: 0.184

Authors:

P.Dhavala,A.C.Papageorgiou

Key ref:

P.Dhavala and a.c.papageorgiou (2009). Structure of helicobacter pylori l-Asparaginase at 1.4 a resolution. Acta crystallogr.,Sect.D, 65,

Date:

11-Sep-09 Release date: 24-Nov-09

Protein chain

Q9ZLB9  (ASPG_HELPJ) -  Probable L-asparaginase

Seq: 332 a.a.

Struc: 315 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.1.1 - Asparaginase.
• Reaction: L-asparagine + H₂O = L-aspartate + NH₃

L-asparagine + H(2)O = L-aspartate (Bound ligand (Het Group name = ASP) corresponds exactly) + NH(3)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: cytoplasm (1 term)
• Biological process: cellular amino acid metabolic process (2 terms)
• Biochemical function: hydrolase activity (2 terms)