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PDBsum entry 2wse

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protein ligands Protein-protein interface(s) links
Photosynthesis PDB id
2wse
Jmol
Contents
Protein chains
164 a.a. *
176 a.a. *
160 a.a. *
166 a.a. *
730 a.a. *
733 a.a. *
81 a.a. *
138 a.a. *
65 a.a. *
154 a.a. *
95 a.a. *
69 a.a. *
30 a.a. *
42 a.a. *
84 a.a. *
161 a.a. *
85 a.a. *
53 a.a. *
Ligands
CLA ×165
LMU ×49
SUC ×13
BCR ×18
PQN ×2
CLA-CLA ×3
LMG
SF4 ×3
CLA-LMU ×2
UNL
* Residue conservation analysis
PDB id:
2wse
Name: Photosynthesis
Title: Improved model of plant photosystem i
Structure: Photosystem i p700 chlorophyll a apoprotein a1. Chain: a. Synonym: psaa, psi-a. Photosystem i p700 chlorophyll a apoprotein a2. Chain: b. Synonym: psab, psi-b. Photosystem i iron-sulfur center. Chain: c. Synonym: psac, photosystem i subunit vii, 9 kda polypeptide
Source: Pisum sativum. Pea. Organism_taxid: 3888. Spinacia oleracea. Spinach. Organism_taxid: 3562. Arabidopsis thaliana. Thale cress. Organism_taxid: 3702.
Resolution:
3.50Å     R-factor:   0.369     R-free:   0.375
Authors: A.Amunts,H.Toporik,A.Borovikov,N.Nelson
Key ref: A.Amunts et al. (2010). Structure determination and improved model of plant photosystem I. J Biol Chem, 285, 3478-3486. PubMed id: 19923216
Date:
05-Sep-09     Release date:   17-Nov-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q01667  (CAB6_ARATH) -  Chlorophyll a-b binding protein 6, chloroplastic
Seq:
Struc:
241 a.a.
164 a.a.
Protein chain
Pfam   ArchSchema ?
Q41038  (Q41038_PEA) -  Type II chlorophyll a/b binding protein from photosystem I
Seq:
Struc:
269 a.a.
176 a.a.*
Protein chain
Pfam   ArchSchema ?
C6TEX2  (C6TEX2_SOYBN) -  Putative uncharacterized protein
Seq:
Struc:
276 a.a.
160 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9SQL2  (CB24_PEA) -  Chlorophyll a-b binding protein P4, chloroplastic
Seq:
Struc:
252 a.a.
166 a.a.
Protein chain
Pfam   ArchSchema ?
P05310  (PSAA_PEA) -  Photosystem I P700 chlorophyll a apoprotein A1
Seq:
Struc:
 
Seq:
Struc:
758 a.a.
730 a.a.
Protein chain
Pfam   ArchSchema ?
P05311  (PSAB_PEA) -  Photosystem I P700 chlorophyll a apoprotein A2
Seq:
Struc:
 
Seq:
Struc:
734 a.a.
733 a.a.
Protein chain
Pfam   ArchSchema ?
P10793  (PSAC_PEA) -  Photosystem I iron-sulfur center
Seq:
Struc:
81 a.a.
81 a.a.
Protein chain
Pfam   ArchSchema ?
P12353  (PSAD_SPIOL) -  Photosystem I reaction center subunit II, chloroplastic
Seq:
Struc:
212 a.a.
138 a.a.
Protein chain
Pfam   ArchSchema ?
Q9S831  (PSAE1_ARATH) -  Photosystem I reaction center subunit IV A, chloroplastic
Seq:
Struc:
143 a.a.
65 a.a.
Protein chain
Pfam   ArchSchema ?
P12355  (PSAF_SPIOL) -  Photosystem I reaction center subunit III, chloroplastic
Seq:
Struc:
231 a.a.
154 a.a.
Protein chain
Pfam   ArchSchema ?
P12357  (PSAG_SPIOL) -  Photosystem I reaction center subunit V, chloroplastic
Seq:
Struc:
167 a.a.
95 a.a.
Protein chain
Pfam   ArchSchema ?
P22179  (PSAH_SPIOL) -  Photosystem I reaction center subunit VI, chloroplastic
Seq:
Struc:
144 a.a.
69 a.a.
Protein chain
Pfam   ArchSchema ?
P17227  (PSAI_PEA) -  Photosystem I reaction center subunit VIII
Seq:
Struc:
40 a.a.
30 a.a.
Protein chain
Pfam   ArchSchema ?
P17230  (PSAJ_SPIOL) -  Photosystem I reaction center subunit IX
Seq:
Struc:
44 a.a.
42 a.a.
Protein chain
Pfam   ArchSchema ?
P36886  (PSAK_HORVU) -  Photosystem I reaction center subunit psaK, chloroplastic
Seq:
Struc:
131 a.a.
84 a.a.*
Protein chain
Pfam   ArchSchema ?
Q41385  (PSAL_SPIOL) -  Photosystem I reaction center subunit XI, chloroplastic
Seq:
Struc:
216 a.a.
161 a.a.
Protein chain
Pfam   ArchSchema ?
Q84U30  (Q84U30_PHAVU) -  Photosystem I-N subunit
Seq:
Struc:
170 a.a.
85 a.a.
Protein chain
No UniProt id for this chain
Struc: 53 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B, C: E.C.1.97.1.12  - Photosystem I.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   14 terms 
  Biological process     divalent metal ion transport   19 terms 
  Biochemical function     electron carrier activity     9 terms  

 

 
J Biol Chem 285:3478-3486 (2010)
PubMed id: 19923216  
 
 
Structure determination and improved model of plant photosystem I.
A.Amunts, H.Toporik, A.Borovikova, N.Nelson.
 
  ABSTRACT  
 
Photosystem I functions as a sunlight energy converter, catalyzing one of the initial steps in driving oxygenic photosynthesis in cyanobacteria, algae, and higher plants. Functionally, Photosystem I captures sunlight and transfers the excitation energy through an intricate and precisely organized antenna system, consisting of a pigment network, to the center of the molecule, where it is used in the transmembrane electron transfer reaction. Our current understanding of the sophisticated mechanisms underlying these processes has profited greatly from elucidation of the crystal structures of the Photosystem I complex. In this report, we describe the developments that ultimately led to enhanced structural information of plant Photosystem I. In addition, we report an improved crystallographic model at 3.3-A resolution, which allows analysis of the structure in more detail. An improved electron density map yielded identification and tracing of subunit PsaK. The location of an additional ten beta-carotenes as well as five chlorophylls and several loop regions, which were previously uninterpretable, are now modeled. This represents the most complete plant Photosystem I structure obtained thus far, revealing the locations of and interactions among 17 protein subunits and 193 non-covalently bound photochemical cofactors. Using the new crystal structure, we examine the network of contacts among the protein subunits from the structural perspective, which provide the basis for elucidating the functional organization of the complex.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21438681 M.F.Hohmann-Marriott, and R.E.Blankenship (2011).
Evolution of photosynthesis.
  Annu Rev Plant Biol, 62, 515-548.  
21344591 M.Sener, J.Strümpfer, J.Hsin, D.Chandler, S.Scheuring, C.N.Hunter, and K.Schulten (2011).
Förster energy transfer theory as reflected in the structures of photosynthetic light-harvesting systems.
  Chemphyschem, 12, 518-531.  
20490922 A.R.Grossman, S.J.Karpowicz, M.Heinnickel, D.Dewez, B.Hamel, R.Dent, K.K.Niyogi, X.Johnson, J.Alric, F.A.Wollman, H.Li, and S.S.Merchant (2010).
Phylogenomic analysis of the Chlamydomonas genome unmasks proteins potentially involved in photosynthetic function and regulation.
  Photosynth Res, 106, 3.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.