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PDBsum entry 2wje

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protein metals links
Hydrolase PDB id
2wje
Jmol
Contents
Protein chain
244 a.a. *
Metals
_MN ×3
Waters ×382
* Residue conservation analysis
PDB id:
2wje
Name: Hydrolase
Title: Crystal structure of the tyrosine phosphatase cps4b from steptococcus pneumoniae tigr4.
Structure: Tyrosine-protein phosphatase cpsb. Chain: a. Synonym: cps4b. Engineered: yes
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Strain: tigr 4. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.90Å     R-factor:   0.141     R-free:   0.190
Authors: G.Hagelueken,H.Huang,J.H.Naismith
Key ref:
G.Hagelueken et al. (2009). Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly. J Mol Biol, 392, 678-688. PubMed id: 19616007 DOI: 10.1016/j.jmb.2009.07.026
Date:
25-May-09     Release date:   14-Jul-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9AHD4  (CPSB1_STRPN) -  Tyrosine-protein phosphatase CpsB
Seq:
Struc:
243 a.a.
244 a.a.
Key:    PfamA domain  Secondary structure

 Enzyme reactions 
   Enzyme class: E.C.3.1.3.48  - Protein-tyrosine-phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate
Protein tyrosine phosphate
+ H(2)O
= protein tyrosine
+ phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     peptidyl-tyrosine dephosphorylation   4 terms 
  Biochemical function     catalytic activity     5 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.jmb.2009.07.026 J Mol Biol 392:678-688 (2009)
PubMed id: 19616007  
 
 
Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly.
G.Hagelueken, H.Huang, I.L.Mainprize, C.Whitfield, J.H.Naismith.
 
  ABSTRACT  
 
Many Gram-positive and Gram-negative bacteria utilize polysaccharide surface layers called capsules to evade the immune system; consequently, the synthesis and export of the capsule are a potential therapeutic target. In Escherichia coli K-30, the integral membrane tyrosine autokinase Wzc and the cognate phosphatase Wzb have been shown to be key for both synthesis and assembly of capsular polysaccharides. In the Gram-positive bacterium Streptococcus pneumoniae, the CpsCD complex is analogous to Wzc and the phosphatase CpsB is the corresponding cognate phosphatase. The phosphatases are known to dephosphorylate their corresponding autokinases, yet despite their functional equivalence, they share no sequence homology. We present the structure of Wzb in complex with phosphate and high-resolution structures of apo-CpsB and a phosphate-complexed CpsB. We show that both proteins are active toward Wzc and thereby demonstrate that CpsB is not specific for CpsCD. CpsB is a novel enzyme and represents the first solved structure of a tyrosine phosphatase from a Gram-positive bacterium. Wzb and CpsB have completely different structures, suggesting that they must operate by very different mechanisms. Although the mechanism of Wzb can be inferred from previous studies, CpsB appears to have a tyrosine phosphatase mechanism not observed before. We propose a chemical mechanism for CpsB based on site-directed mutagenesis and structural data.
 
  Selected figure(s)  
 
Figure 2.
Fig. 2. (a) Crystal structure of Cps4B (stereo pair). The structure is shown in ribbon representation and colored with a gradient running from yellow (N-terminus) to red (C-terminus). The metal ions bound to the active site are shown as purple spheres; metal ligands, in ball-and-stick mode. The alternative conformation of the flexible loop connecting α6/βF is shown in blue. (b) Topology plot of Cps4B. Helices are shown as cylinders; strands, as arrows; and loops, as gray lines. The approximate positions of the metal ions, with respect to the secondary structure elements, are indicated by purple spheres.
Figure 7.
Fig. 7. Product-based alignment of the Wzb[K-30] (yellow) and Cps4B (green) structures. Metals M1, M2 and M3 of Cps4B are depicted as purple spheres. Metal-bound water molecules of Cps4B (W1, W2 and W3) are shown as magenta spheres.
 
  The above figures are reprinted from an Open Access publication published by Elsevier: J Mol Biol (2009, 392, 678-688) copyright 2009.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
  21139210 S.R.Bushell, H.Lou, G.D.Wallat, K.Beis, C.Whitfield, and J.H.Naismith (2010).
Crystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia coli.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 1621-1625.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.