PDBsum entry 2w6f

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protein Protein-protein interface(s) links
Hydrolase PDB id
Protein chains
487 a.a. *
467 a.a. *
122 a.a. *
* Residue conservation analysis
PDB id:
Name: Hydrolase
Title: Low resolution structures of bovine mitochondrial f1-atpase during controlled dehydration: hydration state 2.
Structure: Atp synthase subunit alpha heart isoform, mitochondrial. Chain: a, b, c. Synonym: f1-atpase alpha subunit. Atp synthase subunit beta, mitochondrial. Chain: d, e, f. Synonym: f1-atpase beta subunit. Atp synthase subunit gamma, mitochondrial. Chain: g.
Source: Bos taurus. Bovine. Organism_taxid: 9913. Organ: heart. Tissue: muscle. Organelle: mitochondria. Organelle: mitochonria. Organelle: mitochonria
6.00Å     R-factor:   0.345     R-free:   0.343
Authors: J.Sanchez-Weatherby,F.Felisaz,A.Gobbo,J.Huet,R.B.G.Ravelli, M.W.Bowler,F.Cipriani
Key ref: J.Sanchez-Weatherby et al. (2009). Improving diffraction by humidity control: a novel device compatible with X-ray beamlines. Acta Crystallogr D Biol Crystallogr, 65, 1237-1246. PubMed id: 19966409
18-Dec-08     Release date:   27-Oct-09    
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Protein chains
Pfam   ArchSchema ?
P19483  (ATPA_BOVIN) -  ATP synthase subunit alpha, mitochondrial
553 a.a.
487 a.a.
Protein chains
Pfam   ArchSchema ?
P00829  (ATPB_BOVIN) -  ATP synthase subunit beta, mitochondrial
528 a.a.
467 a.a.
Protein chain
Pfam   ArchSchema ?
P05631  (ATPG_BOVIN) -  ATP synthase subunit gamma, mitochondrial
298 a.a.
122 a.a.
Key:    PfamA domain  Secondary structure

 Enzyme reactions 
   Enzyme class: Chains D, E, F: E.C.  - H(+)-transporting two-sector ATPase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O + H+(In) = ADP + phosphate + H+(Out)
+ H(2)O
+ H(+)(In)
+ phosphate
+ H(+)(Out)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   12 terms 
  Biological process     transport   12 terms 
  Biochemical function     nucleotide binding     10 terms  


Acta Crystallogr D Biol Crystallogr 65:1237-1246 (2009)
PubMed id: 19966409  
Improving diffraction by humidity control: a novel device compatible with X-ray beamlines.
J.Sanchez-Weatherby, M.W.Bowler, J.Huet, A.Gobbo, F.Felisaz, B.Lavault, R.Moya, J.Kadlec, R.B.Ravelli, F.Cipriani.
Dehydration of protein crystals is rarely used, despite being a post-crystallization method that is useful for the improvement of crystal diffraction properties, as it is difficult to reproduce and monitor. A novel device for hydration control of macromolecular crystals in a standard data-collection environment has been developed. The device delivers an air stream of precise relative humidity that can be used to alter the amount of water in macromolecular crystals. The device can be rapidly installed and is fully compatible with most standard synchrotron X-ray beamlines. Samples are mounted in cryoloops and the progress of dehydration can be monitored both optically and by the acquisition of diffraction images. Once the optimal hydration level has been obtained, cryocooling is easy to achieve by hand or by using a sample changer. The device has been thoroughly tested on several ESRF beamlines and is available to users.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21525639 C.Rajendran, F.S.Dworkowski, M.Wang, and C.Schulze-Briese (2011).
Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.
  J Synchrotron Radiat, 18, 318-328.  
21217699 J.Kadlec, E.Hallacli, M.Lipp, H.Holz, J.Sanchez-Weatherby, S.Cusack, and A.Akhtar (2011).
Structural basis for MOF and MSL3 recruitment into the dosage compensation complex by MSL1.
  Nat Struct Mol Biol, 18, 142-149.
PDB codes: 2y0m 2y0n
21976025 N.J.Hu, S.Iwata, A.D.Cameron, and D.Drew (2011).
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
  Nature, 478, 408-411.
PDB codes: 3zux 3zuy
20724792 J.Gabadinho, A.Beteva, M.Guijarro, V.Rey-Bakaikoa, D.Spruce, M.W.Bowler, S.Brockhauser, D.Flot, E.J.Gordon, D.R.Hall, B.Lavault, A.A.McCarthy, J.McCarthy, E.Mitchell, S.Monaco, C.Mueller-Dieckmann, D.Nurizzo, R.B.Ravelli, X.Thibault, M.A.Walsh, G.A.Leonard, and S.M.McSweeney (2010).
MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments.
  J Synchrotron Radiat, 17, 700-707.  
20693684 M.W.Bowler, M.Guijarro, S.Petitdemange, I.Baker, O.Svensson, M.Burghammer, C.Mueller-Dieckmann, E.J.Gordon, D.Flot, S.M.McSweeney, and G.A.Leonard (2010).
Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection.
  Acta Crystallogr D Biol Crystallogr, 66, 855-864.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.