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PDBsum entry 2w5f

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
2w5f
Jmol
Contents
Protein chains
511 a.a. *
Ligands
XYP-XYP-XYP ×2
ACT ×9
Metals
_CD ×13
Waters ×1598
* Residue conservation analysis
PDB id:
2w5f
Name: Hydrolase
Title: High resolution crystallographic structure of the clostridium thermocellum n-terminal endo-1,4-beta-d- xylanase 10b (xyn10b) cbm22-1-gh10 modules complexed with xylohexaose
Structure: Endo-1,4-beta-xylanase y. Chain: a, b. Fragment: cbm22-1, residues 32-551. Synonym: 1\,4-beta-d-xylan xylanohydrolase y, endo-1\,4-beta-d-xylanase 10b, xylanase y, xyly. Engineered: yes. Mutation: yes. Other_details: 2 xylohexaoses (but only 3 of the 6 xylose r be seen in the electron density), 9 acetates, 13 cadmium i
Source: Clostridium thermocellum. Organism_taxid: 1515. Strain: ys. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.90Å     R-factor:   0.166     R-free:   0.194
Authors: S.Najmudin,B.A.Pinheiro,M.J.Romao,J.A.M.Prates,C.M.G.A.Fonte
Key ref: S.Najmudin et al. (2010). Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose. J Struct Biol, 172, 353-362. PubMed id: 20682344 DOI: 10.1016/j.jsb.2010.07.009
Date:
10-Dec-08     Release date:   19-Jan-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P51584  (XYNY_CLOTM) -  Endo-1,4-beta-xylanase Y
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1077 a.a.
511 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.8  - Endo-1,4-beta-xylanase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     carbohydrate metabolic process   1 term 
  Biochemical function     hydrolase activity, acting on glycosyl bonds     2 terms  

 

 
DOI no: 10.1016/j.jsb.2010.07.009 J Struct Biol 172:353-362 (2010)
PubMed id: 20682344  
 
 
Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose.
S.Najmudin, B.A.Pinheiro, J.A.Prates, H.J.Gilbert, M.J.Romão, C.M.Fontes.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20839234 M.F.Lensink, and S.J.Wodak (2010).
Blind predictions of protein interfaces by docking calculations in CAPRI.
  Proteins, 78, 3085-3095.  
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