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PDBsum entry 2w2s
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Viral protein
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PDB id
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2w2s
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Viral protein
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Title:
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Structure of the lagos bat virus matrix protein
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Structure:
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Matrix protein. Chain: a. Synonym: lagos bat virus matrix protein. Engineered: yes
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Source:
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Lagos bat virus. Organism_taxid: 38766. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: isolate 8619nga, genotype 2, isolated from a frugivorous bat in nigeria (boulger, l. R., And j. S. Portefield. 1958. Isolation of a virus from nigerian fruit bats. Trans.R.Soc.Trop.Med.Hyg. 52\:421-424.)
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Resolution:
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2.75Å
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R-factor:
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0.210
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R-free:
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0.255
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Authors:
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S.C.Graham,R.Assenberg,O.Delmas,A.Verma,A.Gholami,C.Talbi,R.J.Owens, D.I.Stuart,J.M.Grimes,H.Bourhy
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Key ref:
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S.C.Graham
et al.
(2008).
Rhabdovirus matrix protein structures reveal a novel mode of self-association.
Plos Pathog,
4,
e1000251.
PubMed id:
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Date:
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03-Nov-08
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Release date:
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13-Jan-09
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PROCHECK
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Headers
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References
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Q6JAM6
(MATRX_LBV) -
Matrix protein from Lagos bat virus
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Seq:
Struc:
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202 a.a.
163 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Plos Pathog
4:e1000251
(2008)
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PubMed id:
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Rhabdovirus matrix protein structures reveal a novel mode of self-association.
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S.C.Graham,
R.Assenberg,
O.Delmas,
A.Verma,
A.Gholami,
C.Talbi,
R.J.Owens,
D.I.Stuart,
J.M.Grimes,
H.Bourhy.
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ABSTRACT
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The matrix (M) proteins of rhabdoviruses are multifunctional proteins essential
for virus maturation and budding that also regulate the expression of viral and
host proteins. We have solved the structures of M from the vesicular stomatitis
virus serotype New Jersey (genus: Vesiculovirus) and from Lagos bat virus
(genus: Lyssavirus), revealing that both share a common fold despite sharing no
identifiable sequence homology. Strikingly, in both structures a stretch of
residues from the otherwise-disordered N terminus of a crystallographically
adjacent molecule is observed binding to a hydrophobic cavity on the surface of
the protein, thereby forming non-covalent linear polymers of M in the crystals.
While the overall topology of the interaction is conserved between the two
structures, the molecular details of the interactions are completely different.
The observed interactions provide a compelling model for the flexible
self-assembly of the matrix protein during virion morphogenesis and may also
modulate interactions with host proteins.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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M.Moerdyk-Schauwecker,
D.Destephanis,
E.Hastie,
and
V.Z.Grdzelishvili
(2011).
Detecting protein-protein interactions in vesicular stomatitis virus using a cytoplasmic yeast two hybrid system.
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J Virol Methods,
173,
203-212.
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M.J.Schnell,
J.P.McGettigan,
C.Wirblich,
and
A.Papaneri
(2010).
The cell biology of rabies virus: using stealth to reach the brain.
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Nat Rev Microbiol,
8,
51-61.
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P.Ge,
J.Tsao,
S.Schein,
T.J.Green,
M.Luo,
and
Z.H.Zhou
(2010).
Cryo-EM model of the bullet-shaped vesicular stomatitis virus.
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Science,
327,
689-693.
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PDB code:
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R.Assenberg,
O.Delmas,
J.Ren,
P.O.Vidalain,
A.Verma,
F.Larrous,
S.C.Graham,
F.Tangy,
J.M.Grimes,
and
H.Bourhy
(2010).
Structure of the nucleoprotein binding domain of Mokola virus phosphoprotein.
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J Virol,
84,
1089-1096.
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PDB code:
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S.Finke,
H.Granzow,
J.Hurst,
R.Pollin,
and
T.C.Mettenleiter
(2010).
Intergenotypic replacement of lyssavirus matrix proteins demonstrates the role of lyssavirus M proteins in intracellular virus accumulation.
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J Virol,
84,
1816-1827.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
