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Oxidoreductase PDB id
2w0x
Jmol
Contents
Protein chain
342 a.a. *
Ligands
PD2
SO4 ×3
GOL
Metals
FE2
Waters ×200
* Residue conservation analysis
PDB id:
2w0x
Name: Oxidoreductase
Title: Factor inhibiting hif-1 alpha with pyridine 2,4 dicarboxylic acid
Structure: Hypoxia-inducible factor 1 alpha inhibitor. Chain: a. Synonym: hypoxia-inducible factor asparagine hydroxylase, factor inhibiting hif-1, fih-1. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.12Å     R-factor:   0.213     R-free:   0.262
Authors: A.Conejo-Garcia,B.M.R.Lienard,I.J.Clifton,M.A.Mcdonough, C.J.Schofield
Key ref: A.Conejo-Garcia et al. (2010). Structural basis for binding of cyclic 2-oxoglutarate analogues to factor-inhibiting hypoxia-inducible factor. Bioorg Med Chem Lett, 20, 6125-6128. PubMed id: 20822901 DOI: 10.1016/j.bmcl.2010.08.032
Date:
10-Oct-08     Release date:   24-Nov-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9NWT6  (HIF1N_HUMAN) -  Hypoxia-inducible factor 1-alpha inhibitor
Seq:
Struc: 		349 a.a.
342 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.1.14.11.16  - Peptide-aspartate beta-dioxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Peptide L-aspartate + 2-oxoglutarate + O2 = peptide 3-hydroxy-L- aspartate + succinate + CO2
Peptide L-aspartate
 +
2-oxoglutarate
Bound ligand (Het Group name = PD2)
matches with 69.00% similarity 		+ O(2)
 = peptide 3-hydroxy-L- aspartate
 + succinate
 + CO(2)
      Cofactor: Iron


Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     nucleus   1 term 
  Biological process     oxidation reduction   3 terms 
  Biochemical function     protein binding     5 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.bmcl.2010.08.032 Bioorg Med Chem Lett 20:6125-6128 (2010)
PubMed id: 20822901  
 
 
Structural basis for binding of cyclic 2-oxoglutarate analogues to factor-inhibiting hypoxia-inducible factor.
A.Conejo-Garcia, M.A.McDonough, C.Loenarz, L.A.McNeill, K.S.Hewitson, W.Ge, B.M.Liénard, C.J.Schofield, I.J.Clifton.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21460794 R.Chowdhury, K.K.Yeoh, Y.M.Tian, L.Hillringhaus, E.A.Bagg, N.R.Rose, I.K.Leung, X.S.Li, E.C.Woon, M.Yang, M.A.McDonough, O.N.King, I.J.Clifton, R.J.Klose, T.D.Claridge, P.J.Ratcliffe, C.J.Schofield, and A.Kawamura (2011).
The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases.
  EMBO Rep, 12, 463-469.
PDB codes: 2ybk 2ybp 2ybs 2yc0 2yde
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.