PDBsum entry: 2vib

Transferase

PDB id: 2vib

Contents

Protein chain

405 a.a. *

Ligands

GLY-PLP

MPD ×2

PO4

Waters ×415

* Residue conservation analysis

PDB id:

2vib

Name:

Transferase

Title:

Crystal structure of s172absshmt obtained in the presence of l-allo-thr

Structure:

Serine hydroxymethyltransferase. Chain: a. Engineered: yes. Mutation: yes

Source:

Geobacillus stearothermophilus. Organism_taxid: 1422. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.85Å R-factor: 0.173 R-free: 0.211

Authors:

V.Rajaram,B.S.Bhavani,V.Prakash,N.Appaji Rao,H.S.Savithri, M.R.N.Murthy

Key ref:

V.Rajaram et al. Crystal structure of s172absshmt and its complexes. To be published,

Date:

28-Nov-07 Release date: 18-Dec-07

Protein chain

Q7SIB6  (Q7SIB6_GEOSE) -  Serine hydroxymethyltransferase

Seq: 419 a.a.

Struc: 405 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.1.2.1 - Glycine hydroxymethyltransferase.
• Pathway: Folate Coenzymes
• Reaction: 5,10-methylenetetrahydrofolate + glycine + H₂O = tetrahydrofolate + L-serine

5,10-methylenetetrahydrofolate + glycine (Bound ligand (Het Group name = GLY) corresponds exactly) + H(2)O = tetrahydrofolate + L-serine

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.00% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: cytoplasm (1 term)
• Biological process: one-carbon metabolic process (4 terms)
• Biochemical function: catalytic activity (4 terms)