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PDBsum entry 2vd6

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protein ligands metals Protein-protein interface(s) links
Lyase PDB id
2vd6
Jmol
Contents
Protein chain
461 a.a. *
Ligands
AMP ×2
FUM ×2
GOL ×5
2SA ×2
Metals
_CL ×7
Waters ×880
* Residue conservation analysis
PDB id:
2vd6
Name: Lyase
Title: Human adenylosuccinate lyase in complex with its substrate n6-(1,2-dicarboxyethyl)-amp, and its products amp and fumarate.
Structure: Adenylosuccinate lyase. Chain: a, b, c, d. Fragment: residues 1-481. Synonym: adenylosuccinase, asl, asase. Engineered: yes. Other_details: the construct contains a hexahistidine tail linker sequence in the n-terminus ( mhhhhhhssgvdlgtenlyfqs
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: mammalian gene collection (mgc)
Resolution:
2.00Å     R-factor:   0.194     R-free:   0.229
Authors: P.Stenmark,M.Moche,C.Arrowsmith,H.Berglund,R.Busam,R.Collins L.G.Dahlgren,A.Edwards,S.Flodin,A.Flores,S.Graslund,M.Hamma B.M.Hallberg,L.Holmberg-Schiavone,I.Johansson,A.Kallas,T.Ka T.Kotenyova,L.Lehtio,M.Nilsson,T.Nyman,D.Ogg,C.Persson,J.Sa M.Sundstrom,A.G.Thorsell,L.Tresaugues,S.Van Den Berg,J.Weig M.Welin,P.Nordlund,Structural Genomics Consortium (Sgc)
Key ref: P.Stenmark et al. Human adenylosuccinate lyase in complex with its substrate n6-(1,2-Dicarboxyethyl)-Amp, And its products amp and fumarate.. To be published, .
Date:
30-Sep-07     Release date:   23-Oct-07    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P30566  (PUR8_HUMAN) -  Adenylosuccinate lyase
Seq:
Struc:
484 a.a.
461 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.3.2.2  - Adenylosuccinate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Purine Biosynthesis (late stages)
      Reaction:
1. N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP
2. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
N(6)-(1,2-dicarboxyethyl)AMP
Bound ligand (Het Group name = 2SA)
corresponds exactly
=
fumarate
Bound ligand (Het Group name = FUM)
corresponds exactly
+
AMP
Bound ligand (Het Group name = AMP)
corresponds exactly
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate
=
fumarate
Bound ligand (Het Group name = FUM)
corresponds exactly
+ 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     mitochondrion   2 terms 
  Biological process     'de novo' AMP biosynthetic process   17 terms 
  Biochemical function     catalytic activity     4 terms