PDBsum entry: 2v40

Ligase

PDB id: 2v40

Contents

Protein chain

419 a.a. *

Ligands

GDP

Waters ×538

* Residue conservation analysis

PDB id:

2v40

Name:

Ligase

Title:

Human adenylosuccinate synthetase isozyme 2 in complex with gdp

Structure:

Adenylosuccinate synthetase isozyme 2. Chain: a. Fragment: residues 21-456. Synonym: adenylosuccinate synthetase acidic isozyme, imp-aspartate ligase 2, adss 2, ampsase 2. Engineered: yes. Other_details: the protein chain contains a hexahistidine tail in the n-terminus

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.166 R-free: 0.198

Authors:

M.Welin,M.Moche,C.Arrowsmith,H.Berglund,R.Busam,R.Collins, L.G.Dahlgren,A.Edwards,S.Flodin,A.Flores,S.Graslund, M.Hammarstrom,B.M.Hallberg,L.Holmberg-Schiavone, I.Johansson,A.Kallas,T.Karlberg,T.Kotenyova,L.Lehtio, T.Nyman,D.Ogg,C.Persson,J.Sagemark,P.Stenmark,M.Sundstrom, A.G.Thorsell,L.Tresaugues,S.Van-Den-Berg,J.Weigelt, P.Nordlund,Structural Genomics Consortium (Sgc)

Key ref:

M.Welin et al. Human adenylosuccinate synthetase isozyme 2 in complex with gdp. To be published,

Date:

27-Jun-07 Release date: 10-Jul-07

Protein chain

P30520  (PURA2_HUMAN) -  Adenylosuccinate synthetase isozyme 2

Seq: 456 a.a.

Struc: 419 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.4.4 - Adenylosuccinate synthase.
• Pathway: AMP and GMP Biosynthesis
• Reaction: GTP + IMP + L-aspartate = GDP + phosphate + N₆-(1,2-dicarboxyethyl)- AMP

GTP + IMP + L-aspartate = GDP (Bound ligand (Het Group name = GDP) corresponds exactly) + phosphate + N(6)-(1,2-dicarboxyethyl)- AMP

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: cellular_component (2 terms)
• Biological process: immune system process (4 terms)
• Biochemical function: nucleotide binding (7 terms)