PDBsum entry 2v0e

Hydrolase

PDB id: 2v0e

Contents

Protein chain

55 a.a. *

* Residue conservation analysis

PDB id:

2v0e

Name:

Hydrolase

Title:

Brk domain from human chd7

Structure:

Chromodomain-helicase-DNA-binding protein 7. Chain: a. Fragment: brk domain, residues 1799-1852. Synonym: atp- dependent helicase chd7, chd-7. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: c41.

NMR struc:

20 models

Authors:

M.D.Allen,T.L.Religa,S.M.V.Freund,M.Bycroft

Key ref:

M.D.Allen et al. (2007). Solution structure of the BRK domains from CHD7. J Mol Biol, 371, 1135-1140. PubMed id: 17603073 DOI: 10.1016/j.jmb.2007.06.007

Date:

14-May-07 Release date: 22-May-07

Protein chain

Q9P2D1  (CHD7_HUMAN) -  Chromodomain-helicase-DNA-binding protein 7 from Homo sapiens

Seq: 2997 a.a.

Struc: 55 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.jmb.2007.06.007

J Mol Biol 371:1135-1140 (2007)

PubMed id: 17603073

Solution structure of the BRK domains from CHD7.

M.D.Allen, T.L.Religa, S.M.Freund, M.Bycroft.

ABSTRACT

CHD7 is a member of the chromodomain helicase DNA binding domain (CHD) family of ATP-dependent chromatin remodelling enzymes. It is mutated in CHARGE syndrome, a multiple congenital anomaly condition. CHD7 is one of a subset of CHD proteins, unique to metazoans that contain the BRK domain, a protein module also found in the Brahma/BRG1 family of helicases. We describe here the NMR solution structure of the two BRK domains of CHD7. Each domain has a compact betabetaalphabeta fold. The second domain has a C-terminal extension consisting of two additional helices. The structure differs from those of other domains present in chromatin-associated proteins.

Selected figure(s)

Figure 3.
Figure 3. CTCF binding. A GST fusion protein of the zinc finger domains of human CTCF (lane 3) bound to glutathione beads was incubated with the BRK domains of CHD7. After repeated washing steps, binding of the BRK domains was analysed by SDS–PAGE followed by Coomassie staining of the gel (lane 4). GST served as a negative control (lanes 5 and 6). For comparison, the BRK input is shown in lane 1. The BRK domains did not bind to glutathione beads (lane 2).

The above figure is reprinted by permission from Elsevier: J Mol Biol (2007, 371, 1135-1140) copyright 2007.