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PDBsum entry 2v01

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protein metals links
Metal-binding protein PDB id
2v01
Jmol
Contents
Protein chain
141 a.a. *
Metals
_CA
_PB ×8
Waters ×55
* Residue conservation analysis
PDB id:
2v01
Name: Metal-binding protein
Title: Recombinant vertebrate calmodulin complexed with pb
Structure: Calmodulin. Chain: a. Synonym: cam. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.15Å     R-factor:   0.191     R-free:   0.253
Authors: P.Kursula,V.Majava
Key ref:
P.Kursula and V.Majava (2007). A structural insight into lead neurotoxicity and calmodulin activation by heavy metals. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 653-656. PubMed id: 17671360 DOI: 10.1107/S1744309107034525
Date:
04-May-07     Release date:   14-Aug-07    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P62158  (CALM_HUMAN) -  Calmodulin
Seq:
Struc:
149 a.a.
141 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   16 terms 
  Biological process     Fc-epsilon receptor signaling pathway   46 terms 
  Biochemical function     enzyme regulator activity     18 terms  

 

 
DOI no: 10.1107/S1744309107034525 Acta Crystallogr Sect F Struct Biol Cryst Commun 63:653-656 (2007)
PubMed id: 17671360  
 
 
A structural insight into lead neurotoxicity and calmodulin activation by heavy metals.
P.Kursula, V.Majava.
 
  ABSTRACT  
 
Calmodulin is a calcium sensor that is also capable of binding and being activated by other metal ions. Of specific interest in this respect is lead, which is known to be neurotoxic and to have a very high affinity towards calmodulin. Crystal structures of human calmodulin complexed with lead and barium ions have been solved. The results will help in understanding the activation mechanisms of calmodulin by different heavy metals and will provide a detailed view of a putative target for lead neurotoxicity in humans.
 
  Selected figure(s)  
 
Figure 1.
Stereoviews of the overall structures of the complexes and the locations of the bound heavy-metal atoms: (a) the Pb complex, (b) the Ba complex. Small green spheres indicate Ca atoms, large magenta spheres indicate soaked heavy-metal atoms bound to the EF-hands and yellow large spheres indicate heavy-metal atoms bound outside the EF-hands. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 August 1; 63(Pt 8): 653–656. Published online 2007 July 28. doi: 10.1107/S1744309107034525. Copyright [copyright] International Union of Crystallography 2007
Figure 2.
Anomalous difference map (cyan) around the bound Ba atoms (magenta) at EF-hand 2 contoured at 5[sigma]. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 August 1; 63(Pt 8): 653–656. Published online 2007 July 28. doi: 10.1107/S1744309107034525. Copyright [copyright] International Union of Crystallography 2007
 
  The above figures are reprinted from an Open Access publication published by the IUCr: Acta Crystallogr Sect F Struct Biol Cryst Commun (2007, 63, 653-656) copyright 2007.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
19404396 V.Majava, and P.Kursula (2009).
Domain swapping and different oligomeric States for the complex between calmodulin and the calmodulin-binding domain of calcineurin a.
  PLoS ONE, 4, e5402.
PDB code: 2w73
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