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RNA-binding protein PDB id
2uy1
Jmol
Contents
Protein chains
438 a.a. *
384 a.a. *
Waters ×320
* Residue conservation analysis
PDB id:
2uy1
Name: RNA-binding protein
Title: Crystal structure of cstf-77
Structure: Cleavage stimulation factor 77. Chain: a. Synonym: similarity to suppressor of forked protein, mRNA stability. Engineered: yes. Cleavage stimulation factor 77. Chain: b. Synonym: similarity to suppressor of forked protein, mRNA stability.
Source: Encephalitozoon cuniculi. Organism_taxid: 6035. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Resolution:
2.0Å     R-factor:   0.227     R-free:   0.280
Authors: P.Legrand,N.Pinaud,L.Minvielle-Sebastia,S.Fribourg
Key ref: P.Legrand et al. (2007). The structure of the CstF-77 homodimer provides insights into CstF assembly. Nucleic Acids Res, 35, 4515-4522. PubMed id: 17584787 DOI: 10.1093/nar/gkm458
Date:
02-Apr-07     Release date:   03-Jul-07    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q8SWC5  (Q8SWC5_ENCCU) -  Similarity to SUPPRESSOR OF FORKED PROTEIN(MRNA stability)
Seq:
Struc: 		493 a.a.
438 a.a.*
Protein chain
Pfam   ArchSchema ?
Q8SWC5  (Q8SWC5_ENCCU) -  Similarity to SUPPRESSOR OF FORKED PROTEIN(MRNA stability)
Seq:
Struc: 		493 a.a.
384 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   2 terms 
  Biological process     RNA processing   2 terms 

 

 
DOI no: 10.1093/nar/gkm458 Nucleic Acids Res 35:4515-4522 (2007)
PubMed id: 17584787  
 
 
The structure of the CstF-77 homodimer provides insights into CstF assembly.
P.Legrand, N.Pinaud, L.Minvielle-Sébastia, S.Fribourg.
 
  ABSTRACT  
 
The cleavage stimulation factor (CstF) is essential for the first step of poly(A) tail formation at the 3' ends of mRNAs. This heterotrimeric complex is built around the 77-kDa protein bridging both CstF-64 and CstF-50 subunits. We have solved the crystal structure of the 77-kDa protein from Encephalitozoon cuniculi at a resolution of 2 A. The structure folds around 11 Half-a-TPR repeats defining two domains. The crystal structure reveals a tight homodimer exposing phylogenetically conserved areas for interaction with protein partners. Mapping experiments identify the C-terminal region of Rna14p, the yeast counterpart of CstF-77, as the docking domain for Rna15p, the yeast CstF-64 homologue.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20967574 C.D.Mackereth (2011).
Chemical shift assignments of a minimal Rna14p/Rna15p heterodimer from the yeast cleavage factor IA complex.
  Biomol NMR Assign, 5, 93-95.  
21481776 M.Moreno-Morcillo, L.Minvielle-Sébastia, S.Fribourg, and C.D.Mackereth (2011).
Locked tether formation by cooperative folding of Rna14p monkeytail and Rna15p hinge domains in the yeast CF IA complex.
  Structure, 19, 534-545.
PDB code: 2l9b
20097654 C.Pancevac, D.C.Goldstone, A.Ramos, and I.A.Taylor (2010).
Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors.
  Nucleic Acids Res, 38, 3119-3132.
PDB codes: 2x1a 2x1b 2x1f
19887456 J.A.Hockert, H.J.Yeh, and C.C.MacDonald (2010).
The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.
  J Biol Chem, 285, 695-704.  
19515729 E.A.Champion, L.Kundrat, L.Regan, and S.J.Baserga (2009).
A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics.
  Protein Eng Des Sel, 22, 431-439.  
19282972 G.A.Palidwor, S.Shcherbinin, M.R.Huska, T.Rasko, U.Stelzl, A.Arumughan, R.Foulle, P.Porras, L.Sanchez-Pulido, E.E.Wanker, and M.A.Andrade-Navarro (2009).
Detection of alpha-rod protein repeats using a neural network and application to huntingtin.
  PLoS Comput Biol, 5, e1000304.  
19217410 Y.Shi, D.C.Di Giammartino, D.Taylor, A.Sarkeshik, W.J.Rice, J.R.Yates, J.Frank, and J.L.Manley (2009).
Molecular architecture of the human pre-mRNA 3' processing complex.
  Mol Cell, 33, 365-376.  
18725399 E.A.Champion, B.H.Lane, M.E.Jackrel, L.Regan, and S.J.Baserga (2008).
A direct interaction between the Utp6 half-a-tetratricopeptide repeat domain and a specific peptide in Utp21 is essential for efficient pre-rRNA processing.
  Mol Cell Biol, 28, 6547-6556.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.