PDBsum entry 2rhl

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protein ligands Protein-protein interface(s) links
Cell cycle PDB id
Protein chains
305 a.a. *
GDP ×2
Waters ×97
* Residue conservation analysis
PDB id:
Name: Cell cycle
Title: Synthetic gene encoded bacillus subtilis ftsz ncs dimer with bound gdp
Structure: Cell division protein ftsz. Chain: a, b. Fragment: residues 12-315. Engineered: yes
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: ftsz. Expressed in: escherichia coli. Expression_system_taxid: 562.
2.45Å     R-factor:   0.229     R-free:   0.270
Authors: S.Lovell,Z.Halloran,K.Hjerrild,D.Sheridan,A.Burgin, L.Stewart,Accelerated Technologies Center For Gene To 3d Structure (Atcg3d)
Key ref: A.Raymond et al. (2009). Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer. BMC Biotechnol, 9, 37. PubMed id: 19383143
09-Oct-07     Release date:   21-Oct-08    
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Protein chains
Pfam   ArchSchema ?
P17865  (FTSZ_BACSU) -  Cell division protein FtsZ
382 a.a.
305 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein complex   2 terms 
  Biological process     protein polymerization   2 terms 
  Biochemical function     GTP binding     2 terms  


BMC Biotechnol 9:37 (2009)
PubMed id: 19383143  
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.
A.Raymond, S.Lovell, D.Lorimer, J.Walchli, M.Mixon, E.Wallace, K.Thompkins, K.Archer, A.Burgin, L.Stewart.
BACKGROUND: With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript. RESULTS: In this report, we compare heterologous protein expression levels from native sequences to that of codon engineered synthetic gene constructs designed by Gene Composer. A test set of proteins including a human kinase (P38alpha), viral polymerase (HCV NS5B), and bacterial structural protein (FtsZ) were expressed in both E. coli and a cell-free wheat germ translation system. We also compare the protein expression levels in E. coli for a set of 11 different proteins with greatly varied G:C content and codon bias. CONCLUSION: The results consistently demonstrate that protein yields from codon engineered Gene Composer designs are as good as or better than those achieved from the synonymous native genes. Moreover, structure guided N- and C-terminal deletion constructs designed with the aid of Gene Composer can lead to greater success in gene to structure work as exemplified by the X-ray crystallographic structure determination of FtsZ from Bacillus subtilis. These results validate the Gene Composer algorithms, and suggest that using a combination of synthetic gene and protein construct engineering tools can improve the economics of gene to structure research.

Literature references that cite this PDB file's key reference

  PubMed id Reference
20037581 A.B.Burgin, O.T.Magnusson, J.Singh, P.Witte, B.L.Staker, J.M.Bjornsson, M.Thorsteinsdottir, S.Hrafnsdottir, T.Hagen, A.S.Kiselyov, L.J.Stewart, and M.E.Gurney (2010).
Design of phosphodiesterase 4D (PDE4D) allosteric modulators for enhancing cognition with improved safety.
  Nat Biotechnol, 28, 63-70.
PDB codes: 3g45 3g4g 3g4i 3g4k 3g4l 3g58 3iad
20700447 S.Yamada, M.Hatta, B.L.Staker, S.Watanabe, M.Imai, K.Shinya, Y.Sakai-Tagawa, M.Ito, M.Ozawa, T.Watanabe, S.Sakabe, C.Li, J.H.Kim, P.J.Myler, I.Phan, A.Raymond, E.Smith, R.Stacy, C.A.Nidom, S.M.Lank, R.W.Wiseman, B.N.Bimber, D.H.O'Connor, G.Neumann, L.J.Stewart, and Y.Kawaoka (2010).
Biological and structural characterization of a host-adapting amino acid in influenza virus.
  PLoS Pathog, 6, 0.
PDB codes: 3kc6 3khw 3l56
19383142 D.Lorimer, A.Raymond, J.Walchli, M.Mixon, A.Barrow, E.Wallace, R.Grice, A.Burgin, and L.Stewart (2009).
Gene composer: database software for protein construct design, codon engineering, and gene synthesis.
  BMC Biotechnol, 9, 36.  
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