PDBsum entry: 2rhk

Viral protein/nuclear protein

PDB id: 2rhk

Contents

Protein chains

119 a.a.

63 a.a. *

60 a.a. *

Ligands

NO3 ×5

TRS

Metals

_ZN ×4

Waters ×185

* Residue conservation analysis

PDB id:

2rhk

Name:

Viral protein/nuclear protein

Title:

Crystal structure of influenza a ns1a protein in complex with f2f3 fragment of human cellular factor cpsf30, northeast structural genomics targets or8c and hr6309a

Structure:

Non-structural protein 1. Chain: a, b. Fragment: ns1a effector domain (unp residues 85-215). Synonym: ns1, ns1a. Engineered: yes. Cleavage and polyadenylation specificity factor subunit 4. Chain: c, d. Fragment: f2f3 zinc-binding domains (unp residues 61-121).

Source:

Influenza a virus. Organism_taxid: 11320. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human. Organism_taxid: 9606.

Resolution:

1.95Å R-factor: 0.210 R-free: 0.234

Authors:

K.Das,L.-C.Ma,R.Xiao,B.Radvansky,J.Aramini,L.Zhao,E.Arnold, R.M.Krug,G.T.Montelione,Northeast Structural Genomics Consortium (Nesg)

Date:

09-Oct-07 Release date: 01-Jul-08

Protein chains

P03495  (NS1_I72A2) -  Non-structural protein 1

Seq: 237 a.a.

Struc: 119 a.a.

Protein chain

O95639  (CPSF4_HUMAN) -  Cleavage and polyadenylation specificity factor subunit 4

Seq: 269 a.a.

Struc: 63 a.a.*

Protein chain

O95639  (CPSF4_HUMAN) -  Cleavage and polyadenylation specificity factor subunit 4

Seq: 269 a.a.

Struc: 60 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 

• Biochemical function: nucleic acid binding (3 terms)