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PDBsum entry 2rhh

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Cell cycle PDB id
2rhh
Jmol
Contents
Protein chain
304 a.a. *
Ligands
SO4
Waters ×114
* Residue conservation analysis
PDB id:
2rhh
Name: Cell cycle
Title: Synthetic gene encoded bacillus subtilis ftsz with bound sulfate ion
Structure: Cell division protein ftsz. Chain: a. Fragment: residues 12-315. Engineered: yes
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: ftsz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.00Å     R-factor:   0.215     R-free:   0.257
Authors: S.Lovell,Z.Halloran,K.Hjerrild,D.Sheridan,A.Burgin, L.Stewart,Accelerated Technologies Center For Gene To 3d Structure (Atcg3d)
Key ref: A.Raymond et al. (2009). Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer. BMC Biotechnol, 9, 37. PubMed id: 19383143
Date:
09-Oct-07     Release date:   21-Oct-08    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P17865  (FTSZ_BACSU) -  Cell division protein FtsZ
Seq:
Struc:
382 a.a.
304 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein complex   2 terms 
  Biological process     protein polymerization   2 terms 
  Biochemical function     GTP binding     2 terms  

 

 
BMC Biotechnol 9:37 (2009)
PubMed id: 19383143  
 
 
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.
A.Raymond, S.Lovell, D.Lorimer, J.Walchli, M.Mixon, E.Wallace, K.Thompkins, K.Archer, A.Burgin, L.Stewart.
 
  ABSTRACT  
 
BACKGROUND: With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript. RESULTS: In this report, we compare heterologous protein expression levels from native sequences to that of codon engineered synthetic gene constructs designed by Gene Composer. A test set of proteins including a human kinase (P38alpha), viral polymerase (HCV NS5B), and bacterial structural protein (FtsZ) were expressed in both E. coli and a cell-free wheat germ translation system. We also compare the protein expression levels in E. coli for a set of 11 different proteins with greatly varied G:C content and codon bias. CONCLUSION: The results consistently demonstrate that protein yields from codon engineered Gene Composer designs are as good as or better than those achieved from the synonymous native genes. Moreover, structure guided N- and C-terminal deletion constructs designed with the aid of Gene Composer can lead to greater success in gene to structure work as exemplified by the X-ray crystallographic structure determination of FtsZ from Bacillus subtilis. These results validate the Gene Composer algorithms, and suggest that using a combination of synthetic gene and protein construct engineering tools can improve the economics of gene to structure research.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20037581 A.B.Burgin, O.T.Magnusson, J.Singh, P.Witte, B.L.Staker, J.M.Bjornsson, M.Thorsteinsdottir, S.Hrafnsdottir, T.Hagen, A.S.Kiselyov, L.J.Stewart, and M.E.Gurney (2010).
Design of phosphodiesterase 4D (PDE4D) allosteric modulators for enhancing cognition with improved safety.
  Nat Biotechnol, 28, 63-70.
PDB codes: 3g45 3g4g 3g4i 3g4k 3g4l 3g58 3iad
20700447 S.Yamada, M.Hatta, B.L.Staker, S.Watanabe, M.Imai, K.Shinya, Y.Sakai-Tagawa, M.Ito, M.Ozawa, T.Watanabe, S.Sakabe, C.Li, J.H.Kim, P.J.Myler, I.Phan, A.Raymond, E.Smith, R.Stacy, C.A.Nidom, S.M.Lank, R.W.Wiseman, B.N.Bimber, D.H.O'Connor, G.Neumann, L.J.Stewart, and Y.Kawaoka (2010).
Biological and structural characterization of a host-adapting amino acid in influenza virus.
  PLoS Pathog, 6, 0.
PDB codes: 3kc6 3khw 3l56
19383142 D.Lorimer, A.Raymond, J.Walchli, M.Mixon, A.Barrow, E.Wallace, R.Grice, A.Burgin, and L.Stewart (2009).
Gene composer: database software for protein construct design, codon engineering, and gene synthesis.
  BMC Biotechnol, 9, 36.  
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